首页|STRAP binds to and promotes the repair of N1-methyldeoxyadenosine in DNA

STRAP binds to and promotes the repair of N1-methyldeoxyadenosine in DNA

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N1-methyladenosine(m1A)is an important RNA modification that functions in various biological pro-cesses by interacting with cellular proteins.However,the binding proteins of N1-methyldeoxyadenosine(1mdA)in DNA remain largely unknown.Herein,we employed a quantitative proteomics strategy to iden-tify the potential binding proteins of 1mdA in human cells.Our results revealed that serine-threonine kinase receptor-associated protein(STRAP)can bind to 1mdA-carrying DNA.We further demonstrated that STRAP participates in alkylating agent-induced DNA damage response and can promote the repair of 1mdA embedded in DNA.Moreover,we investigated the effects of STRAP on 1mdA-induced perturba-tion in transcription using a shuttle vector-and next-generation sequencing-based assay,and found that STRAP is involved in the transcriptional bypass of 1mdA in human cells.Together,our study revealed STRAP as a novel 1mdA-binding protein in human cells and provided new insight into the biological implications of STRAP and 1mdA modification in human diseases.

N1-methyldeoxyadenosine1mdA-binding proteinSTRAPDNA repairTranscription

Meijuan Zhou、Qian Liu、Mengting Pan、Ying Yang、Xiaoxia Dai、Changjun You

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State Key Laboratory of Chemo/Biosensing and Chemometrics,Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology,Molecular Science and Biomedicine Laboratory,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China

国家自然科学基金国家自然科学基金湖南省自然科学基金Science and Technology Innovation Program of Hunan Province中央高校基本科研业务费专项中央高校基本科研业务费专项

21907028218070302023JJ301192019RS2020531118010061531118010259

2024

10.1016/j.cclet.2023.108673

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(3)
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