Abstract
N1-methyladenosine(m1A)is an important RNA modification that functions in various biological pro-cesses by interacting with cellular proteins.However,the binding proteins of N1-methyldeoxyadenosine(1mdA)in DNA remain largely unknown.Herein,we employed a quantitative proteomics strategy to iden-tify the potential binding proteins of 1mdA in human cells.Our results revealed that serine-threonine kinase receptor-associated protein(STRAP)can bind to 1mdA-carrying DNA.We further demonstrated that STRAP participates in alkylating agent-induced DNA damage response and can promote the repair of 1mdA embedded in DNA.Moreover,we investigated the effects of STRAP on 1mdA-induced perturba-tion in transcription using a shuttle vector-and next-generation sequencing-based assay,and found that STRAP is involved in the transcriptional bypass of 1mdA in human cells.Together,our study revealed STRAP as a novel 1mdA-binding protein in human cells and provided new insight into the biological implications of STRAP and 1mdA modification in human diseases.
基金项目
国家自然科学基金(21907028)
国家自然科学基金(21807030)
湖南省自然科学基金(2023JJ30119)
Science and Technology Innovation Program of Hunan Province(2019RS2020)
中央高校基本科研业务费专项(531118010061)
中央高校基本科研业务费专项(531118010259)
NETL
NSTL
万方数据