首页|A lateral-immobilization zebrafish microfluidic chip-based system for in vivo real-time evaluation of antithrombotic agents

A lateral-immobilization zebrafish microfluidic chip-based system for in vivo real-time evaluation of antithrombotic agents

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Thrombosis remains a major global health concern mainly characterized by high rates of morbidity and mortality.Animal models serve as an indispensable tool to understand the underlying pathogenesis of thrombosis and assess the efficacy of novel antithrombotic drugs.Currently,zebrafish has emerged as a valuable model organism for thrombosis research.However,the traditional method of studying zebrafish thrombosis requires a laborious and time-consuming procedure,including anesthesia and manual im-mobilization of zebrafish.In this study,based on hydrodynamic force,a lateral-immobilization zebrafish microfluidic chip(LIZMC)was designed to evaluate the cardiovascular system of multiple larvae within a single microscope field of view.Specifically,coupling with microscope imaging,real-time monitoring of the peripheral blood circulation in the tail of phenylhydrazine(PHZ)-induced zebrafish thrombosis was enabled.Furthermore,the reliability of LIZMC for in vivo evaluation of antithrombotic agents in zebrafish was verified using aspirin.Collectively,this novel LIZMC-based system can be used for in vivo zebrafish thrombosis studies and rapid screening of antithrombotic agents.

Microfluidic chipZebrafishThrombosisIn vivo evaluationReal-time monitor

Lijuan He、Hongxia Du、Yi Yang、Zhihua Guan、Jinjin Li、Honglin Li、Xudong Lin、Lili Zhu

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Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science & Technology,Shanghai 200237,China

Innovation Center for AI and Drug Discovery,East China Normal University,Shanghai 200062,China

Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument,School of Biomedical Engineering,Shenzhen Campus of Sun Yat-sen University,Shenzhen 518000,China

国家自然科学基金国家自然科学基金Shanghai Science and Technology Commission Biomedical science and Technology Support Special ProjectShanghai Science and Technology Commission Biomedical science and Technology Support Special Project中央高校基本科研业务费专项Shanghai"Beyond Limits Manufacturing"ProjectNational Program for Special Supports of Eminent ProfessionalsNational Program for Support of Topnotch Young Professionals

818250208215020821S1190790020S11901000

2024

10.1016/j.cclet.2023.109013

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(3)
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