Applying dynamic light scattering to investigate the self-assembly process of DNA nanostructures

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外文摘要：Understanding the dynamic assembly process of DNA nanostructures is important for developing novel strategy to design and construct functional devices.In this work,temperature-controlled dynamic light scattering(DLS)strategy has been applied to study the global assembly process of DNA origami and DNA bricks.Through the temperature dependent size and intensity profiles,the self-assembly process of various DNA nanostructures with different morphologies have been well-studied and the temperature transition ranges could be observed.Taking advantage of the DLS information,rapid preparation of the DNA origami and the brick assembly has been realized through a constant temperature annealing.Our results demonstrate that the DLS-based strategy provides a convenient and robust tool to study the dy-namic process of forming hieratical DNA structures,which will benefit understanding the mechanism of self-assembly of DNA nanostructures.

外文关键词：

Dynamic light scatteringSelf-assembly processDNA nanostructuresTemperature transition rangesRapid preparation

作者：

Wei Yuan、Gui-Zhi Dong、Hui Ning、Xiang-Xiang Guan、Jia-Feng Cheng、Zi-Wei Shi、Xiu-Ji Du、Si-Wen Meng、Dong-Sheng Liu、Yuan-Chen Dong

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作者单位：

CAS Key Laboratory of Colloid,Interface and Chemical Thermodynamics,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China

University of Chinese Academy of Sciences,Beijing 100049,China

Dandong Bettersize Instruments Ltd.,Dandong 118000,China

Key Laboratory of Organic Optoelectronics & Molecular Engineering of the Ministry of Education,Department of Chemistry,Tsinghua University,Beijing 100084,China

e Department of Chemistry,Renmain University of China,Beijing 100872,China

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基金：

国家自然科学基金

项目编号：

21971248

出版年：

2024

DOI：

10.1016/j.cclet.2023.108384

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

年,卷(期)：2024.35(3)

参考文献量43