首页|Synthesis and potential osteogenic applications of Wnt3a-loaded ZIF-8 nanoparticles

Synthesis and potential osteogenic applications of Wnt3a-loaded ZIF-8 nanoparticles

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
The Wnt signaling pathway plays a critical role in bone homeostasis,and the related protein therapy strategies have been reported to have great potential in osseointegration;however,they face formidable challenges such as complex external environments and unavoidable protein denaturation.In this work,we report a novel approach combining the synthesis of metal-organic frameworks(MOFs)and protein encapsulation in a one-pot process based on zeolitic imidazolate framework-8(ZIF-8)and Wnt3a protein,with improved biomechanical behavior and enhanced protein biological response.This combination was designed to enhance the Wnt3a protein function through the improved chemical stability provided by the ZIF-8 crystals.Additionally,the zinc ions contained in the ZIF-8 crystals induced bone homeostasis,further favoring the osteogenesis.The results showed that the Wnt3a protein-loaded ZIF-8 crystals served as efficient drug delivery vehicles to promote osteogenesis,preventing protein denaturation.In particular,Wnt3a-loaded ZIF-8 nanoparticles(Wnt3a@ZIF-8 NPs)had higher efficacy on bone marrow mesenchymal stem cells(BMSCs)than ZIF-8 NPs or Wnt3a proteins,contributing to the osteogenesis through ZIF-8 crystals and intracellular Wnt3a proteins released from Wnt3a@ZIF-8 NPs.Furthermore,polymerase chain reaction(PCR)analysis showed that the osteogenic pathways were upregulated.Overall,the present one-pot process can open up new avenues to develop signaling protein-delivery systems for applications in protein therapy strategies.

Metal-organic frameworksNanoparticleDrug releaseOsteogenesisBone defects

Hengfei Wang、Song Chen、Zihan He、Junyu Chen、Zhou Zhu、Qianbing Wan、Jian Wang、Xibo Pei

展开 >

State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases,Department of Prosthodontics,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China

Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education,Stomatological Hospital of Chongqing Medical University,Chongqing 400016,China

国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金Key research program of Sichuan Science and technology DepartmentResearch and Development Program(West China Hospital of Stomatology,Sichuan University)Research and Development Program(West China Hospital of Stomatology,Sichuan University)

822710168227103482270961820711648197032972819010602021YFS0052RD-03-202107RD-03-202310

2024

10.1016/j.cclet.2023.108597

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(3)
  • 44