Abstract
Aging is a natural physiological process with various challenges,related to the loss of homeostasis within the organism,which is not a disease,but a significantly strong risk factor for multiple diseases,including myocardial infarction,stroke,some age-related cancers,macular degeneration,osteoarthritis,neurode-generation,and many others.In the body,the main manifestation of aging is cellular aging,which exists within tissues and has a local or global impact on tissue function.However,the lack of effective aging detection tools has always been an issue that cannot be ignored in the field of aging research.Therefore,it is necessary to construct a non-invasive tool for in vivo detection of aging.Here,we show that the photoacoustic probe(LGAL),which has peak excitation and emission wavelengths in the near-infrared optical window,binds in vivo and at high contrast to the hallmark of aging,and allows for the micro-scopic imaging of aging through the intact mice.Firstly,this tool LGAL has been successfully applied to detect senescence in cells,displaying stronger photoacoustic signals than normal cells.Then,by using the photoacoustic probe,the blood vessels and tissues inside the mice can be visualized.Young and elderly mice exhibit varying intensities of photoacoustic signals,marking the first time a probe has been used to explore the aging of blood vessels and tissues inside the mice.Finally,we monitored the changes in the degree of aging during tumor treatment under photoacoustic(PA)imaging for the first time.As the treatment time increased,the degree of aging of the tumor gradually deepened.We expect the powerful tool could be a noninvasive and powerful tool for the study of aging biology.
基金项目
国家自然科学基金(21877048)
国家自然科学基金(22077048)
国家自然科学基金(22277014)
广西自然科学基金(2021GXNSFDA075003)
广西自然科学基金(AD21220061)
startup fund of Guangxi University(A3040051003)
NETL
NSTL
万方数据