Abstract
The reactive oxygen species(ROS)generation efficiency is always limited by the extreme tumor mi-croenvironment(TME),leading to unsatisfactory antitumor effects in photodynamic therapy(PDT).As a promising gas therapy molecule,nitric oxide(NO)is independent of oxygen and could even synergize ROS to enhance the therapeutic effect.However,the short half-life,instability,and uncontrollable release of exogenous NO limited the application of tumor synergistic therapy.Herein,we reported a novel kind of red-emissive carbon dots(CDs)that was capable of lysosome-targeted and light-controlled NO deliv-ery.The CDs were synthesized by using metformin and methylene blue(MB)via a hydrothermal method.The obtained metformin-MB CDs(MMCDs)exhibited a higher 1O2 quantum yield and NO generation efficiency under light emitting diode(LED)light irradiation.Noteworthily,the 1O2 could further in situ oxidize NO into peroxynitrite anions(ONOO-),which own the higher cytotoxicity against cancer cells.Cell experiments indicate that MMCDs could destruct lysosome membrane integrity and kill almost 80％of HepG2 cells under light irradiation while very low cytotoxicity in the dark.Moreover,MMCDs signif-icantly decreased tumor volume and weight after phototherapy in hepatoma HepG2-bearing mice.Our study provides a new strategy for light-controlled NO generation as well as precise lysosome-targeting for enhancement of PDT efficiency.
基金项目
国家自然科学基金(52172033)
国家自然科学基金(22005280)
support of the Key Labora-tory of Structure and Functional Regulation of Hybrid Materials of the Ministry of Education,Anhui()
Key Laboratory of Environ-ment Friendly Polymer Materials of Anhui Province,China()
Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province,Anhui University,China()
NETL
NSTL
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