首页|Chemoselective labeling-based spermatozoa glycan imaging reveals abnormal glycosylation in oligoasthenotspermia
Chemoselective labeling-based spermatozoa glycan imaging reveals abnormal glycosylation in oligoasthenotspermia
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Spermatogenesis,maturation,capacitation and fertilization are precisely regulated by glycosylation.How-ever,the relationship between altered glycosylation patterns and the onset and development of repro-ductive disorders is unclear,mainly limited by the lack of in situ imaging techniques for spermatozoa glycosylation.We developed an efficient and highly specific spermatozoa glycan imaging technique based on the robust chemoselective labeling of sialic acid(Sia)and N-acetyl-D-galactosamine(Gal/GalNAc).We further proposed a"tandem glycan chemoselective labeling"strategy to achieve simultaneous imaging of two types of glycans on spermatozoa.We applied the developed method to the spermatozoa from oligo-zoospermic patients and diabetic mice and found that these spermatozoa showed higher levels of Sia and Gal/GalNAc expression than the normal groups.Moreover,spermatozoa from diabetic mice showed a severe decrease in number,viability,and forward motility,suggesting that in vivo glucose metabolism disorders may lead to an elevated level of spermatozoa glycosylation and have a correlation with the development of oligoasthenotspermia.Our work provides a research tool to reveal the relationship be-tween glycosylation modification and spermatozoa quality,and a promising clue for the development of glycan-based reproductive markers.
Lijia Xu、Tong Zhong、Wei Zhao、Bing Yao、Lin Ding、Huangxian Ju
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State Key Laboratory of Analytical Chemistry for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,China
Department of Reproductive Medical Center,Jinling Hospital,School of Medicine,Nanjing University,Nanjing 210002,China
Center of Clinical Laboratory Science,The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research,Nanjing 210009,China
Chemistry and Biomedicine Innovation Center(ChemBIC),Nanjing University,Nanjing 210023,China
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国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金National Key Research and Devel-opment Program of China中央高校基本科研业务费专项中央高校基本科研业务费专项中央高校基本科研业务费专项State Key Laboratory of Analytical Chemistry for Life ScienceState Key Laboratory of Analytical Chemistry for Life Science
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