首页|Visible-light-activated aziridination reaction enables simultaneous resolving of C=C bond location and the sn-position isomers in lipids

Visible-light-activated aziridination reaction enables simultaneous resolving of C=C bond location and the sn-position isomers in lipids

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There is a close relationship between the biological functions of lipids and their structures,and vari-ous isomers greatly increases the complexity of lipid structures.The C=C bond location and sn-position are two of the essential attributes that determine the structures of unsaturated lipids.However,simul-taneous identification of both attributes remains challenging.Here,we develop a visible-light-activated aziridination reaction system,which enables the dual-resolving of the C=C bond location and sn-position isomerism of in lipids when combines with liquid chromatography-mass spectrometry(LC-MS).Based on the derivatization of C=C bonds with PhI=NTs,their location in lipids could be easily identified by tandem MS.Especially,the sn-position isomers of unsaturated phosphatidylcholine(PC)can be separated and quantified by LC-MS after the derivatization.By using the proposed method,the significant changes of the sn-position isomers ratios of PC in mouse brain ischemia were revealed.This study offers a pow-erful tool for deep lipid structural biology.

AziridinationLipid isomersC=C bond locationsn-PositionMass spectrometry

Qiongqiong Wan、Yanan Xiao、Guifang Feng、Xin Dong、Wenjing Nie、Ming Gao、Qingtao Meng、Suming Chen

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The Institute for Advanced Studies,Renmin Hospital of Wuhan University,Wuhan University,Wuhan 430072,China

国家自然科学基金国家自然科学基金国家自然科学基金National Key Research and Development Pro-gram of Chinasupport of the start-up funds of Wuhan UniversityNational Youth Talents Plan of China

2207411122004093220040922021YFC2700700

2024

10.1016/j.cclet.2023.108775

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(4)
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