Abstract
There is a close relationship between the biological functions of lipids and their structures,and vari-ous isomers greatly increases the complexity of lipid structures.The C=C bond location and sn-position are two of the essential attributes that determine the structures of unsaturated lipids.However,simul-taneous identification of both attributes remains challenging.Here,we develop a visible-light-activated aziridination reaction system,which enables the dual-resolving of the C=C bond location and sn-position isomerism of in lipids when combines with liquid chromatography-mass spectrometry(LC-MS).Based on the derivatization of C=C bonds with PhI=NTs,their location in lipids could be easily identified by tandem MS.Especially,the sn-position isomers of unsaturated phosphatidylcholine(PC)can be separated and quantified by LC-MS after the derivatization.By using the proposed method,the significant changes of the sn-position isomers ratios of PC in mouse brain ischemia were revealed.This study offers a pow-erful tool for deep lipid structural biology.
基金项目
国家自然科学基金(22074111)
国家自然科学基金(22004093)
国家自然科学基金(22004092)
National Key Research and Development Pro-gram of China(2021YFC2700700)
support of the start-up funds of Wuhan University()
National Youth Talents Plan of China()
NETL
NSTL
万方数据