Abstract
Modern chromatography is increasingly focused on miniaturization and integration.Compared to conven-tional liquid chromatography,microfluidic chip liquid chromatography(microchip-LC)has the potential due to its zero-dead volume connection and ease of integration.Nano-sized packings have the poten-tial to significantly enhance separation performance in microchip-LC.However,their application has been hindered by packing difficulties.This study presents a method for packing nano-sized silica particles into a microchannel as the stationary phase.The microchip-LC packed column was prepared by combining the weir and the porous silica single-particle as frit to retain the packing particles.A surface tension-based single-particle picking technique was established to insert porous single-particle frit into glass microchannels.Additionally,we developed a slurry packing method that utilizes air pressure to inject nano-sized packing into the microchannel.Pressure-driven chromatographic separation was performed using this nano-packed column integrated into a glass microchip.The mixture of four PAHs was success-fully separated within just 8 min using a 5 mm separation channel length,achieving high theoretical plates(106 plates/m).Overall,these findings demonstrate the potential of utilizing nano-sized packings for enhancing chromatographic performance in microchip systems.
基金项目
国家自然科学基金(21936001)
Beijing Outstanding Young Scientist Program(BJJWZYJH01201910005017)
NETL
NSTL
万方数据