首页|Nano-sized stationary phase packings retained by single-particle frit for microchip liquid chromatography
Nano-sized stationary phase packings retained by single-particle frit for microchip liquid chromatography
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
Modern chromatography is increasingly focused on miniaturization and integration.Compared to conven-tional liquid chromatography,microfluidic chip liquid chromatography(microchip-LC)has the potential due to its zero-dead volume connection and ease of integration.Nano-sized packings have the poten-tial to significantly enhance separation performance in microchip-LC.However,their application has been hindered by packing difficulties.This study presents a method for packing nano-sized silica particles into a microchannel as the stationary phase.The microchip-LC packed column was prepared by combining the weir and the porous silica single-particle as frit to retain the packing particles.A surface tension-based single-particle picking technique was established to insert porous single-particle frit into glass microchannels.Additionally,we developed a slurry packing method that utilizes air pressure to inject nano-sized packing into the microchannel.Pressure-driven chromatographic separation was performed using this nano-packed column integrated into a glass microchip.The mixture of four PAHs was success-fully separated within just 8 min using a 5 mm separation channel length,achieving high theoretical plates(106 plates/m).Overall,these findings demonstrate the potential of utilizing nano-sized packings for enhancing chromatographic performance in microchip systems.
Center Excellence for Environmental Safety and Biological Effects,Beijing Key Laboratory for Green Catalysis and Separation,Department of Chemistry,Beijing University of Technology,Beijing 100124,China
Center for Environmental Metrology,National Institute of Metrology,Beijing 100029,China
国家自然科学基金Beijing Outstanding Young Scientist Program
NETL
NSTL
万方数据
