首页|Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis

Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis

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RNA modifications have been involved in numerous biological processes,and aberrations of these mod-ifications are tightly associated with various diseases including cancer.Herein,we developed graphene-based solid-phase extraction and robust ultra performance liquid chromatography-tandem mass spec-trometry(UPLC-MS/MS)combined with stable isotope-dilution for simultaneous enrichment and accurate determination of 17 modified nucleosides in human urine.We found graphene could effectively adsorb various modified nucleosides in human urine samples.With this method,we identified and quantified these modified nucleosides in urine samples collected from lung cancer patients and healthy controls.We revealed that the levels of 12 modified nucleosides were all diminished in urine from lung cancer patients,compared with healthy controls.It is worth noting that we demonstrated,for the first time,the presence of 5,2'-O-dimethyluridine(m5Um)in human urine.Together,we established a robust analytical method for simultaneous determinations of 17 modified nucleosides in human urine,and our results re-vealed a close correlation between the concentrations of urinary modified nucleosides and the occurrence of lung cancer,implying the potential applications of these modified nucleosides as noninvasive biomark-ers for the early detection of lung cancer.Moreover,this study will stimulate future investigations on the regulatory roles of RNA modifications in the initiation and progression of lung cancer.

Mass spectrometryRNA modificationModified nucleosidesGrapheneBiomarkerUrine

Cheng Guo、Xiaoxiao Zhang、Xiujuan Hong、Yiqiu Hu、Lingna Mao、Kezhi Jiang

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Cancer Institute(Key Laboratory of Cancer Prevention and Intervention,China National Ministry of Education),The Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,China

Cancer Center,Zhejiang University,Hangzhou 310058,China

Key Laboratory of Organosilicon Chemistry and Material Technology,College of Material Chemistry and Chemical Engineering,Hangzhou Normal University,Hangzhou 311121,China

Department of General Practice,The Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,China

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国家自然科学基金中央高校基本科研业务费专项浙江省重点研发计划

22176167226-2023-000882021C03125

2024

10.1016/j.cclet.2023.108867

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(4)
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