首页|Site-specific incorporation of reduction-controlled guest amino acids into proteins for cucurbituril recognition
Site-specific incorporation of reduction-controlled guest amino acids into proteins for cucurbituril recognition
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Protein recognition using host-guest recognition approach is of great interest but has been limited mainly to the protein N-terminal residues.Here,we site-specific incorporated two novel non-canonical amino acids containing supramolecular guest motifs into protein via an expanded genetic code.Through Staudinger reduction reactions,the encoded unnatural residues on protein becoming activated and can be specifically recognized by cucurbit[7]uril(CB[7])and cucurbit[8]uril(CB[8]).We demonstrated that enzyme containing guest amino acid incorporated near the active site can be reversibly regulated by CB[7]recognition,and CB[8]recognition induces protein dimerization.These amino acids will make use-ful addition to the supramolecular toolbox for protein targeting using molecular recognition approaches.
Protein recognitionHost-guest recognitionNon-canonical amino acidsStaudinger reductionExpanded genetic code
Zhen Dai、Linzhi Tan、Yeyu Su、Kerui Zhao、Yushun Tian、Yu Liu、Tao Liu
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College of Chemistry,State Key Laboratory of Elemento-Organic Chemistry,Nankai University,Tianjin 300071,China
State Key Laboratory of Natural and Biomimetic Drugs,Department of Molecular and Cellular Pharmacology,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China
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