AbnI:An α-ketoglutarate-dependent dioxygenase involved in brassicicene C-H functionalization and ring system rearrangement

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外文摘要：We reported the characterization of a novel brassicicene diterpene biosynthetic gene cluster,which con-tains a unique α-ketoglutarate-dependent dioxygenase(αKGD)enzyme,AbnI.Our findings revealed that AbnI demonstrates remarkable substrate promiscuity and is capable of activating multiple sites on both 5-8-5 and 5-9-5 brassicicene skeletons,resulting in skeleton modifications and an unexpected ring sys-tem rearrangement.These results suggested the potential utility of AbnI as an enzymatic tool for terpene C-H functionalization.In addition,the catalytic mechanism of AbnI and its potential ecological implica-tions were discussed.

外文关键词：

Fungal diterpenesα-Ketoglutarate-dependent dioxygenaseC-H functionalizationRearrangementBrassicicenes

作者：

Wenling Yuan、Fengli Li、Zhe Chen、Qiaoxin Xu、Zhenhua Guan、Nanyu Yao、Zhengxi Hu、Junjun Liu、Yuan Zhou、Ying Ye、Yonghui Zhang

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作者单位：

Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

基金：

国家重点研发计划国家重点研发计划国家自然科学基金国家自然科学基金国家自然科学基金Program for Changjiang Scholars of Ministry of Education of the People's Republic of China国家杰出青年科学基金National Science and Technology Project of China国家自然科学基金中央高校基本科研业务费专项

项目编号：

2021YFA09105002021YFA0910503222770353200004581973205T2016088817250212018ZX09201001-001-003817210052020kfyXJJS043

出版年：

2024

DOI：

10.1016/j.cclet.2023.108788

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

年,卷(期)：2024.35(5)

参考文献量24