Abstract
We reported the characterization of a novel brassicicene diterpene biosynthetic gene cluster,which con-tains a unique α-ketoglutarate-dependent dioxygenase(αKGD)enzyme,AbnI.Our findings revealed that AbnI demonstrates remarkable substrate promiscuity and is capable of activating multiple sites on both 5-8-5 and 5-9-5 brassicicene skeletons,resulting in skeleton modifications and an unexpected ring sys-tem rearrangement.These results suggested the potential utility of AbnI as an enzymatic tool for terpene C-H functionalization.In addition,the catalytic mechanism of AbnI and its potential ecological implica-tions were discussed.
基金项目
国家重点研发计划(2021YFA0910500)
国家重点研发计划(2021YFA0910503)
国家自然科学基金(22277035)
国家自然科学基金(32000045)
国家自然科学基金(81973205)
Program for Changjiang Scholars of Ministry of Education of the People's Republic of China(T2016088)
国家杰出青年科学基金(81725021)
National Science and Technology Project of China(2018ZX09201001-001-003)
国家自然科学基金(81721005)
中央高校基本科研业务费专项(2020kfyXJJS043)
万方数据