Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex

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外文摘要：Strand displacement reaction is a crucial component in the assembly of diverse DNA-based nanodevices,with the toehold-mediated strand displacement reaction representing the prevailing strategy.However,the single-stranded Watson-Crick sticky region that serves as the trigger for strand displacement can also cause leakage reactions by introducing crosstalk in complex DNA circuits.Here,we proposed the toeless and reversible DNA strand displacement reaction based on the Hoogsteen-bond triplex,which is compat-ible with most of the existing DNA circuits.We demonstrated that our proposed reaction can occur at pH 5 and can be reversed at pH 9.We also observed an approximately linear relationship between the degree of reaction and pH within the range of pH 5-6,providing the potential for precise regulation of the reaction.Meanwhile,by altering the sequence orientation,we have demonstrated that our proposed reaction can be initiated or regulated through the same toeless mechanism without the requirement for protonation in low pH conditions.Based on the proposed reaction principle,we further constructed a variety of DNA nanodevices,including two types of DNA logic gates that rely on pH 5/pH 9 changes for initiating and reversing:the AND gate and the OR gate.We also successfully constructed a DNA Walker based on our proposed reaction modes,which can move along a given track after the introduction of a programmable DNA sequence and complete a cycle after 4 steps.Our findings suggest that this innova-tive approach will have broad utility in the development of DNA circuits,molecular sensors,and other complex biological systems.

外文关键词：

DNA strand displacementToelessTriplex DNA strandsReversibleDNA circuitDNA walker

作者：

Yang Qin、Jiangtian Li、Xuehao Zhang、Kaixuan Wan、Heao Zhang、Feiyang Huang、Limei Wang、Hongxun Wang、Longjie Li、Xianjin Xiao

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作者单位：

Institute of Reproductive Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

Department of Pancreatic Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

Wuhan Polytechnic University,Wuhan 430023,China

基金：

国家重点研发计划Open Research Fund of State Key Laboratory of Bioelectronics,southeast UniversityOpen Foundation of NHC Key Laboratory of Birth Defect for Research and Prevention(Hunan Provincial Maternal and Child HealthOpen Foundation of Translational Medicine National Science and Technology Infrastructure(Shanghai)

项目编号：

2021YFC2701402Sklb2021-k06KF2020007TMSK-2021-141

出版年：

2024

DOI：

10.1016/j.cclet.2023.108826

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

年,卷(期)：2024.35(5)

参考文献量30