首页|Utilization of hydralazine as a reactive matrix for enhanced detection and on-MALDI-target derivatization of saccharides
Utilization of hydralazine as a reactive matrix for enhanced detection and on-MALDI-target derivatization of saccharides
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Saccharides are a sort of ubiquitous and vital molecules within the whole life.However,the application of saccharides analysis with matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)is restricted by their low ionization efficiency and the instability of the sialic acid fraction.Derivatization strategy based on nonreductive amination provides a good solution,however,this is often time consum-ing and may result in sample loss due to removal of excessive derivatization reagents.Herein,hydralazine(HZN)was utilized as a reactive matrix for labeling reducing saccharides directly on MALD1 target which eliminated tedious sample preparation and avoided sample loss.After optimization,effective and repro-ducible on-MALDI-target derivatization of neutral and acidic saccharides was achieved in both positive and negative modes.Compared with 2,5-dihydroxybenzoic acid(DHB)and 9-aminoacridine(9-AA),HZN improved the detection sensitivity of reducing saccharides and provided more abundant fragment ions in MS/MS analysis.Moreover,26 kinds of neutral glycans and 5 kinds of sialic glycans were identified from ovalbumin(OVA)and bovine fetuin,respectively.Combined with the statistical models,this strat-egy could be used to distinguish and predict samples of 6 brands of beer,and discriminate 2 kinds of beer fermentation modes.In addition,HZN was applied for quantitative analysis of glucose in urine samples,and the obtained urine glucose concentrations of diabetic patients were consistent with the clinical test results,showing the potential of qualitative and quantitative analysis of reducing saccharides in complex samples.
Dan Ouyang、Huan Huang、Yanting He、Jiajing Chen、Jiali Lin、Zhuling Chen、Zongwei Cai、Zian Lin
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Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology,Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety,College of Chemistry,Fuzhou University,Fuzhou 350108,China
School of Pharmacy,Bengbu Medical University,Bengbu 233000,China
Partner State Key Laboratory of Environmental and Biological Analysis,Department of Chemistry,Hong Kong Baptist University,Hong Kong,China
国家自然科学基金国家自然科学基金国家自然科学基金福建省自然科学基金Major Project of Science and Technology of Fujian Province
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