Abstract
Saccharides are a sort of ubiquitous and vital molecules within the whole life.However,the application of saccharides analysis with matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)is restricted by their low ionization efficiency and the instability of the sialic acid fraction.Derivatization strategy based on nonreductive amination provides a good solution,however,this is often time consum-ing and may result in sample loss due to removal of excessive derivatization reagents.Herein,hydralazine(HZN)was utilized as a reactive matrix for labeling reducing saccharides directly on MALD1 target which eliminated tedious sample preparation and avoided sample loss.After optimization,effective and repro-ducible on-MALDI-target derivatization of neutral and acidic saccharides was achieved in both positive and negative modes.Compared with 2,5-dihydroxybenzoic acid(DHB)and 9-aminoacridine(9-AA),HZN improved the detection sensitivity of reducing saccharides and provided more abundant fragment ions in MS/MS analysis.Moreover,26 kinds of neutral glycans and 5 kinds of sialic glycans were identified from ovalbumin(OVA)and bovine fetuin,respectively.Combined with the statistical models,this strat-egy could be used to distinguish and predict samples of 6 brands of beer,and discriminate 2 kinds of beer fermentation modes.In addition,HZN was applied for quantitative analysis of glucose in urine samples,and the obtained urine glucose concentrations of diabetic patients were consistent with the clinical test results,showing the potential of qualitative and quantitative analysis of reducing saccharides in complex samples.
基金项目
国家自然科学基金(22274021)
国家自然科学基金(21974021)
国家自然科学基金(22036001)
福建省自然科学基金(2022J01535)
Major Project of Science and Technology of Fujian Province(2020HZ06006)
维普
万方数据