首页|Mobile mini-fluorimeter for antibiotic aptasensing based on surface-plasmonic effect of burlike nanogolds enhanced by digitized imaging diagnosis
Mobile mini-fluorimeter for antibiotic aptasensing based on surface-plasmonic effect of burlike nanogolds enhanced by digitized imaging diagnosis
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Antibiotic abuse now poses a grave threat to global ecology and bestirs public concerns about the residue issue in daily necessities.The traceability measurements along supply chain or logistic circulation have become increasingly essential given the labile nature of diverse synthetic residuals on site.In an attempt to answer this urgency,here a miniaturized fluorometric aptasensor prototype was contrived that catered to the point-of-care screening norm for two typical additives:chloramphenicol and enrofloxacin.The key target-indicating module worked in vitro based on the competitive binding-induced fluorescence recovery of fluorescein-labeled aptamers,which were photobleached beforehand in the format of double helix on burlike nanogold carriers.The"prickly"geometry of the latter not just enriched the capture probes at preferentially substrate-accessible spires;but also contributed to a tip-enhanced surface plasmon effect,sensitizing the signal-on during the duplex dissociation even at nanomolar threshold of the analytes.On the other hand,to encompass a full portable,a set of optical devices were mounted within a 3D-printed cartridge(adaptor)to converge the light beam and route it towards the detector,for which the smartphone camera came up in handy with a home-developed App for calibrating the emissive bright-ness.Enlightened by the high-dynamic-range compression,an imaging diagnostic algorithm was built in to grid and digitize each slide in the album for augmented detection performance.Thus,a novel bio-to-silico integration was invented that capable of in situ rapid reporting on the antibiotic presence with high sensitivity and selectivity.Further field practices in spiked milk on sales proved the precision and rudi-mentary feasibility of the well-assembled model of appliance,thus holding nice prospects in nonexpert(e.g.,family and local community)utilities for foodborne antibiotic identification.
Chloramphenicol and enrofloxacinAptasensorGold nanobursSmartphone fluorimetric suiteDigitized image postprocessing
Tiantian Man、Fulin Zhu、Yaqi Huang、Yuhao Piao、Yan Su、Shengyuan Deng、Ying Wan
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School of Mechanical Engineering,Nanjing University of Science and Technology,Nanjing 210094,China
Key Laboratory of Metabolic Engineering and Biosynthesis Technology,Ministry of Industry and Information Technology,School of Environmental and Biological Engineering,Nanjing University of Science and Technology,Nanjing 210094,China
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