A mitochondria-targeted H2S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury

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外文摘要：Hepatic ischemia-reperfusion injury(HIRI)is the cause of postoperative hepatic dysfunction and failure,and even death.As an important biological effector molecule,hydrogen sulfide(H2S)of mitochondria as a gasotransmitter that is usually used to protect against acute HIRI injury.However,the exact rela-tionship between HIRI and mitochondrial H2S remains tangled due to the lack of an effective analytical method.Herein,we have fabricated a mitochondria-targeted H2S-activatable fluorogenic probe(Mito-GW)to explore the stability of mitochondrial H2S and track the changes of mitochondrial H2S during the HIRI.By virtue of pyridinium electropositivity and its amphiphilicity,Mito-GW could accumulate in mitochon-dria.It goes through an analyte-prompted immolation when reacts with H2S,resulting in the releasing of the fluorophore(GW).Therefore,the extent of Mito-GW conversion to GW can be used to evaluate the changes of mitochondrial H2S level in living cells and tissues.As proof-of-principle,we have used Mito-GW to demonstrate the mitochondria H2S-levels increase and then decrease during HIRI in vitro and in vivo.Our research highlights the tremendous potential of Mito-GW as a mitochondrial H2S fluorogenic probe in elucidating the pathogenesis of HIRI,providing a powerful tool for promoting future research on hepatology.

外文关键词：

Mitochondria-targetedBioimagingH2S-activatableFluorogenic probeHepatic ischemia-reperfusion injury(HIRI)

作者：

Bin Fang、Jiaqi Yang、Limin Wang、Haoqin Li、Jiaying Guo、Jiaxin Zhang、Qingyuan Guo、Bo Peng、Kedi Liu、Miaomiao Xi、Hua Bai、Li Fu、Lin Li

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作者单位：

School of Materials Science and Engineering,Northwestern Polytechnical University,Xi'an 710072,China

Frontiers Science Center for Flexible Electronics,Xi'an Institute of Flexible Electronics(IFE)and Xi'an Institute of Biomedical Materials & Engineering,Northwestern Polytechnical University,Xi'an 710072,China

Key laboratory of Flexible Electronics of Zhejiang Province,Ningbo Institute of Northwestern Polytechnical University,Ningbo 315103,China

Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility,Huazhong University of Science and Technology,Wuhan 430074,China

TANK Medicinal Biology Institute of Xi'an,Xi'an 710032,China

The Institute of Flexible Electronics(IFE,Future Technologies),Xiamen University,Xiamen 361005,China

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基金：

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaJoint Research Funds of Department of Science & Technology of Shaanxi Province and Northwestern Polytechnical UniversityJoint Research Funds of Department of Science & Technology of Shaanxi Province and Northwestern Polytechnical UniversityJoint Research Funds of Department of Science & Technology of Shaanxi Province and Northwestern Polytechnical UniversityNatural Science Foundation of Shaanxi ProvinceNatural Science Foundation of NingboNatural Science Foundation of NingboOpen Project Program of Wuhan National Laboratory for OptoelectronicsOpen Project Program of Wuhan National Laboratory for OptoelectronicsInnovation Foundation for Doctor Dissertation of Northwestern Polytechnical UniversityInnovation Capability Support Program of Shaanxi

项目编号：

22077101220040992020GXLH-Z-0082020GXLH-Z-0212020GXLH-Z-0232022JM-130202003N4049202003N40652020WNLOKF0232022WN-LOKF009CX20220342023-CX-PT-23

出版年：

2024

DOI：

10.1016/j.cclet.2023.108913

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

年,卷(期)：2024.35(6)