A mitochondria-targeted H2S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury

Bin Fang ¹Jiaqi Yang ²Limin Wang ²Haoqin Li ²Jiaying Guo ²Jiaxin Zhang ²Qingyuan Guo ²Bo Peng ³Kedi Liu ⁴Miaomiao Xi ⁴Hua Bai ⁵Li Fu ⁶Lin Li⁷

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作者信息

1. School of Materials Science and Engineering,Northwestern Polytechnical University,Xi'an 710072,China;Frontiers Science Center for Flexible Electronics,Xi'an Institute of Flexible Electronics(IFE)and Xi'an Institute of Biomedical Materials & Engineering,Northwestern Polytechnical University,Xi'an 710072,China
2. Frontiers Science Center for Flexible Electronics,Xi'an Institute of Flexible Electronics(IFE)and Xi'an Institute of Biomedical Materials & Engineering,Northwestern Polytechnical University,Xi'an 710072,China
3. Frontiers Science Center for Flexible Electronics,Xi'an Institute of Flexible Electronics(IFE)and Xi'an Institute of Biomedical Materials & Engineering,Northwestern Polytechnical University,Xi'an 710072,China;Key laboratory of Flexible Electronics of Zhejiang Province,Ningbo Institute of Northwestern Polytechnical University,Ningbo 315103,China;Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility,Huazhong University of Science and Technology,Wuhan 430074,China
4. TANK Medicinal Biology Institute of Xi'an,Xi'an 710032,China
5. Frontiers Science Center for Flexible Electronics,Xi'an Institute of Flexible Electronics(IFE)and Xi'an Institute of Biomedical Materials & Engineering,Northwestern Polytechnical University,Xi'an 710072,China;Key laboratory of Flexible Electronics of Zhejiang Province,Ningbo Institute of Northwestern Polytechnical University,Ningbo 315103,China
6. School of Materials Science and Engineering,Northwestern Polytechnical University,Xi'an 710072,China
7. Frontiers Science Center for Flexible Electronics,Xi'an Institute of Flexible Electronics(IFE)and Xi'an Institute of Biomedical Materials & Engineering,Northwestern Polytechnical University,Xi'an 710072,China;The Institute of Flexible Electronics(IFE,Future Technologies),Xiamen University,Xiamen 361005,China;Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility,Huazhong University of Science and Technology,Wuhan 430074,China
折叠

Abstract

Hepatic ischemia-reperfusion injury(HIRI)is the cause of postoperative hepatic dysfunction and failure,and even death.As an important biological effector molecule,hydrogen sulfide(H2S)of mitochondria as a gasotransmitter that is usually used to protect against acute HIRI injury.However,the exact rela-tionship between HIRI and mitochondrial H2S remains tangled due to the lack of an effective analytical method.Herein,we have fabricated a mitochondria-targeted H2S-activatable fluorogenic probe(Mito-GW)to explore the stability of mitochondrial H2S and track the changes of mitochondrial H2S during the HIRI.By virtue of pyridinium electropositivity and its amphiphilicity,Mito-GW could accumulate in mitochon-dria.It goes through an analyte-prompted immolation when reacts with H2S,resulting in the releasing of the fluorophore(GW).Therefore,the extent of Mito-GW conversion to GW can be used to evaluate the changes of mitochondrial H2S level in living cells and tissues.As proof-of-principle,we have used Mito-GW to demonstrate the mitochondria H2S-levels increase and then decrease during HIRI in vitro and in vivo.Our research highlights the tremendous potential of Mito-GW as a mitochondrial H2S fluorogenic probe in elucidating the pathogenesis of HIRI,providing a powerful tool for promoting future research on hepatology.

Key words

Mitochondria-targeted/Bioimaging/H2S-activatable/Fluorogenic probe/Hepatic ischemia-reperfusion injury(HIRI)

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基金项目

National Natural Science Foundation of China(22077101)

National Natural Science Foundation of China(22004099)

Joint Research Funds of Department of Science & Technology of Shaanxi Province and Northwestern Polytechnical University(2020GXLH-Z-008)

Joint Research Funds of Department of Science & Technology of Shaanxi Province and Northwestern Polytechnical University(2020GXLH-Z-021)

Joint Research Funds of Department of Science & Technology of Shaanxi Province and Northwestern Polytechnical University(2020GXLH-Z-023)

Natural Science Foundation of Shaanxi Province(2022JM-130)

Natural Science Foundation of Ningbo(202003N4049)

Natural Science Foundation of Ningbo(202003N4065)

Open Project Program of Wuhan National Laboratory for Optoelectronics(2020WNLOKF023)

Open Project Program of Wuhan National Laboratory for Optoelectronics(2022WN-LOKF009)

Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University(CX2022034)

Innovation Capability Support Program of Shaanxi(2023-CX-PT-23)

出版年

2024

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

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