Abstract
Elevated level of hypochlorous acid(HClO)is closely associated with cancer development.Identifying HClO level in cancer cells would provide important evidence in either early-stage cancer diagnostics or monitoring of its treatment efficiency.In this work,a new pyronine-based fluorescent probe for rapid and sensitive detection of HClO was developed by condensing meso-formyl pyronine(PyCHO)with 2-hydrazinopyridine to form meso-pyridylhydrazone-functionalized pyronine PyHP,PyHP is nonfluorescent due to the excited-state C=N isomerization nonradiative decay,whereas the HClO-triggered formation of meso-triazolopyridyl pyronine PyTP abolishes the C=N isomerization and thus greatly enhances the fluorescence.With the probe,the cancer cells/tumor were distinguished with high-contrast from normal ones by laser confocal fluorescence imaging,and the tumor-to-normal(T/N)ratios obtained exceed the clinically acceptable threshold of 2.0.Moreover,its capability of in vivo imaging tumor was also demon-strated.These results indicate the potential of PyHP as an effective tool in the early clinical diagnosis of cancers.
基金项目
Scientific and Technological Innovation Program of Colleges and Universities in Shanxi Province(2021L529)
Scientific and Technological Innovation Program of Colleges and Universities in Shanxi Province(2021L530)
Fund for Shanxi"1331 Project"()
NETL
NSTL
万方数据