首页|4-Nitrocatechol as a novel matrix for low-molecular-weight compounds in situ detection and imaging in biological tissues by MALDI-MSI

4-Nitrocatechol as a novel matrix for low-molecular-weight compounds in situ detection and imaging in biological tissues by MALDI-MSI

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Low-molecular-weight(LMW)compounds are ubiquitous in living organisms and play essential roles in biological processes.The direct analysis of LMW compounds in biological tissues by matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI)could provide a more comprehensive un-derstanding of their essential functions.Here,we evaluated 4-nitrocatechol(4-NC)as a novel positive-ion matrix for enhancing in situ detection and imaging of LMW compounds from the rat liver,brain,and germinating Chinese-yew seed by MALD1-MS.Our results showed that the 4-NC possessed remarkable features,including strong ultraviolet absorption,uniform matrix crystal,excellent chemical stability,and fewer matrix-related background peaks.The use of 4-NC led to the successful detection of 232,218,and 193 LMW compounds from the three abovementioned tissue sections,respectively.Also,the use of 4-NC improved the imaging quality of LMW compounds in tissue sections through MALDI-MSI and has the potential as a matrix for MALDI tissue imaging of LMW compounds.

MALDI-MSI4-NitrocatecholLMW compoundsIn situ detectionTissue imaging

Hualei Xu、Manman Han、Haiqiang Liu、Liang Qin、Lulu Chen、Hao Hu、Ran Wu、Chenyu Yang、Hua Guo、Jinrong Li、Jinxiang Fu、Qichen Hao、Yijun Zhou、Jinchao Feng、Xiaodong Wang

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Key Laboratory of Mass Spectrometry Imaging and Metabolomics(Minzu University of China),State Ethnic Affairs Commission,Beijing 100081,China

College of Life and Environmental Sciences,Centre for Imaging & Systems Biology,Minzu University of China,Beijing 100081,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaYouth Academic Team Project of MUCHuayi Technology Innovation Center for Research ResourcesKey Laboratory Construction Funds of State Ethnic Affairs Commission of China

317703842160516410301-02200301HTIC P01RR2017001A10301-02200303

2024

10.1016/j.cclet.2023.109095

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(6)
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