首页|Au-Fe3O4 dumbbell-like nanoparticles based lateral flow immunoassay for colorimetric and photothermal dual-mode detection of SARS-CoV-2 spike protein

Au-Fe3O4 dumbbell-like nanoparticles based lateral flow immunoassay for colorimetric and photothermal dual-mode detection of SARS-CoV-2 spike protein

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Lateral flow immunoassay(LFIA)has become popular in laboratories,at-home testing,and medical diag-nostics due to its minimal cost and user-friendliness.Nevertheless,conventional test strips based on col-loidal gold can only obtain qualitative or semi-quantitative results with low sensitivity.In this work,Au-Fe3O4 dumbbell-like nanoparticles were synthesized and used as the LFIA labelling marker for highly sen-sitive colorimetric-photothermal dual-mode detection of SARS-CoV-2 spike(S)protein.The unique dumb-bell structure of Au-Fe3O4 NPs makes it possible to combine the best features of both Au NPs and Fe3O4 NPs.The increased surface area of these NPs enhances their LSPR effect and photothermal effect,which achieves signal amplification to increase sensitivity.The Au-Fe3O4 NPs modified with S protein antibody could identify S protein in samples,which were recognized and accumulated on T-line by another an-tibody,generating color band for qualitative colorimetric detection.The T-line was irradiated by laser to obtain temperature change for quantitative detection of photothermal.In optimized conditions,the detec-tion limit was 1.22 pg/mL,three orders of magnitude more sensitive than colorimetric detection.Finally,the approach was performed on SARS-CoV-2 pseudovirus samples and outperformed traditional colloidal gold strips.This LFIA platform exhibits significant promise for practical implementation,as it can satisfy the need for low-cost,high-sensitivity,and home-based quantitative detection for respiratory infectious diseases.

Au-Fe3O4 dumbbell-like nanoparticlesPhotothermal effectLFIAS protein

Gengchen Guo、Tianyu Zhao、Ruichang Sun、Mingzhe Song、Hongyu Liu、Sen Wang、Jingwen Li、Jingbin Zeng

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College of Chemistry and Chemical Engineering and State Key Laboratory of heavy oil processing,China University of Petroleum(East China),Qingdao 266580,China

Huangdao Customs of the People's Republic of China,Qingdao 266580,China

Natural Scientific Foundation of ShandongNatural Scientific Foundation of ShandongNational Natural Science Foundation of ChinaFundamental Research Funds for the Central UniversitiesTaishan Scholarship of Shandong Province

ZR2023MC039ZR2022JQ072187620621CX06014Atsqn202211080

2024

10.1016/j.cclet.2023.109198

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(6)