首页|Allosteric DNAzyme-based encoder for molecular information transfer

Allosteric DNAzyme-based encoder for molecular information transfer

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Dynamic DNA nanotechnology plays a significant role in nanomedicine and information science due to its high programmability based on Watson-Crick base pairing and nanoscale dimensions.Intelligent DNA machines and networks have been widely used in various fields,including molecular imaging,biosen-sors,drug delivery,information processing,and logic operations.Encoders serve as crucial components for information compilation and transfer,allowing the conversion of information from diverse application scenarios into a format recognized and applied by DNA circuits.However,there are only a few encoder designs with DNA outputs.Moreover,the molecular priority encoder is hardly designed.In this study,we introduce allosteric DNAzyme-based encoders for information transfer.The design of the allosteric do-main and the recognition arm allows the input and output to be independent of each other and freely programmable.The pre-packaged mode design achieves uniformity of baseline dynamics and dynamics controllability.We also integrated non-nucleic acid molecules into the encoder through the aptamer de-sign of the allosteric domain.Furthermore,we developed the 2n-n encoder and the Endo Ⅳ-assisted pri-ority encoder inspired by immunoglobulin's molecular structure and effector patterns.To our knowledge,the proposed encoder is the first enzyme-free DNA encoder with DNA output,and the priority encoder is the first molecular priority encoder in the DNA reaction network.Our encoders avoid complex operations on a single molecule,and their simple structure facilitates their application in complex DNA circuits and biological scenarios.

DNAzymeEncoderNucleic acidsDNA circuitDNA strand displacementDynamic DNA nanotechnology

Minghao Hu、Tianci Xie、Yuqiang Hu、Longjie Li、Ting Wang、Tongbo Wu

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School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

Department of Oncology,Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

GI Cancer Research Institute,Tongji Hospital,Huazhong University of Science and Technology,Wuhan 430030,China

School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China

State Key Laboratory of Bioelectronics,National Demonstration Center for Experimental Biomedical Engineering Education,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China

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National Natural Science Foundation of ChinaOpening Research Fund of State Key Laboratory of Digital Medical Engineering

821723722023-M04

2024

10.1016/j.cclet.2023.109232

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(7)