Abstract
Tumor microenvironment(TME)-activatable probes have been proven to effectively increase signal-to-background ratios(SBRs)and improve the success rate of complete tumor resection.However,many fluo-rescence probes have to be loaded into a nanocarrier for tumor targeted delivery,which consequently en-counters poor drug loading,heterogeneous composition and non-encapsulated drug aggregates occurred during nanoformulation fabrications.Herein,a nitroreductase(NTR)-activated"OFF-ON"near-infrared flu-orescence nanoprobe,named NanoBodipy,was synthesized by the spontaneous self-assembling of NTR-responsive dye-polyethylene glycol(PEG)amphiphilic polymer in water.The NTR-responsive dye acted as the hydrophobic segment in the amphiphilic polymer,yielding a homogeneous composition and a high loading of 12.2 wt% (according to calculation)in the synthesized NanoBodipy.The synthesized NanoBod-ipy can efficiently accumulate in tumors via the enhanced permeability and retention(EPR)effect,en-abling non-invasive tumor-targeted fluorescence imaging and guiding complete tumor resection.Once the synthesized NanoBodipy entered the tumor cells,they dissociated and were activated by overexpressed NTR.With the real-time fluorescence guide of NanoBodipy,complete tumor resection surgery was per-formed successfully.
基金项目
National Natural Science Foundation of China(51903201)
National Natural Science Foundation of China(82172798)
Natural Science Foundation of Shaanxi Province(2023-YBSF-270)
Open Program of NHC Key Laboratory of Nuclear Medicine and Jiangsu Key Laboratory of Molecular Nuclear Medicine(KF202203)
Horizontal Project of the First Affiliated Hospital of Xi'an Jiaotong University(202304174)
维普
万方数据