Abstract
With the increasing emergence of bacterial infections,especially multidrug-resistant(MDR)bacteria,poses an urgent threat.This study demonstrated a novel multifunctional nanotheranostics platform de-veloped by the strategic integration of both in-situ bio-assembly imaging and target bacteria inacti-vation.Through the introduction of copper ions into bacteria,the Cu2+could spontaneously bio-self-assembled into a multifunctional copper nanoclusters(NCs)which efficiently enhanced epigallocatechin gallate(EGCG)uptake into bacteria.While visualizing the bacteria,the developed theranostic nanoplat-form exhibited highly efficient disinfection activities with negligible side effects as reflected by higher cell viability and insignificant hemolytic effects.Furthermore,the exosomal formulation of EGCG inte-grated with Cu2+showed an increased intracellular antibacterial activity,which could eliminate most of the methicillin-resistant Staphylococcus aureus(MRSA)phagocytosed by macrophages,guide macrophages toward M2-like phenotype polarization and alleviate inflammation,without exhibiting obvious cytotox-icity on host RAW264.7.The regimen could be viewed as an effective strategy for the sterilization of intractable bacterial infections.
基金项目
National Natural Science Foundation of China(82061148012)
National Natural Science Foundation of China(82027806)
National Natural Science Foundation of China(92061121)
National Natural Science Foundation of China(91753106)
National Key Research and Development Program of China(2017YFA0205300)
Primary Research&Development Plan of Jiangsu Province(BE2019716)
ISF-NSFC Joint Research Program(3258/20)
维普
万方数据