首页|Engineered exosome hybrid copper nanoscale antibiotics facilitate simultaneous self-assembly imaging and elimination of intracellular multidrug-resistant superbugs

Engineered exosome hybrid copper nanoscale antibiotics facilitate simultaneous self-assembly imaging and elimination of intracellular multidrug-resistant superbugs

扫码查看
原文链接
  • 万方数据
  • 维普
With the increasing emergence of bacterial infections,especially multidrug-resistant(MDR)bacteria,poses an urgent threat.This study demonstrated a novel multifunctional nanotheranostics platform de-veloped by the strategic integration of both in-situ bio-assembly imaging and target bacteria inacti-vation.Through the introduction of copper ions into bacteria,the Cu2+could spontaneously bio-self-assembled into a multifunctional copper nanoclusters(NCs)which efficiently enhanced epigallocatechin gallate(EGCG)uptake into bacteria.While visualizing the bacteria,the developed theranostic nanoplat-form exhibited highly efficient disinfection activities with negligible side effects as reflected by higher cell viability and insignificant hemolytic effects.Furthermore,the exosomal formulation of EGCG inte-grated with Cu2+showed an increased intracellular antibacterial activity,which could eliminate most of the methicillin-resistant Staphylococcus aureus(MRSA)phagocytosed by macrophages,guide macrophages toward M2-like phenotype polarization and alleviate inflammation,without exhibiting obvious cytotox-icity on host RAW264.7.The regimen could be viewed as an effective strategy for the sterilization of intractable bacterial infections.

In situ self-assembledExosomeAntibacterial agentsBioimagingIntracellular infection

Zengchao Guo、Weiwei Liu、Tengfei Liu、Jinpeng Wang、Hui Jiang、Xiaohui Liu、Yossi Weizmann、Xuemei Wang

展开 >

State Key Laboratory of Digital Medical Engineering,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China

Department of Chemistry,Ben-Gurion University of the Negev,Beer-Sheva 8410501,Israel

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaPrimary Research&Development Plan of Jiangsu ProvinceISF-NSFC Joint Research Program

820611480128202780692061121917531062017YFA0205300BE20197163258/20

2024

10.1016/j.cclet.2023.109060

中国化学快报(英文版)
中国化学会

中国化学快报(英文版)

CSTPCD
影响因子：0.771
ISSN：1001-8417
年,卷(期)：2024.35(7)