Abstract
To overcome the conflict between the long-wavelength excitation and high singlet oxygen quantum yield of photosensitizers,we conjugated a two-photon fluorophore,tetrahydroquinoxaline coumarin(TQ),and an efficient photodynamic therapeutic agent,benzo[a]phenothiazinium(NBS-NH2),through a hexamethy-lene linker to build a two-photon photosensitizer,TQ-NBS.In TQ-NBS,TQ served as an energy donor and NBS-NH2 acted as an energy acceptor;and TQ-NBS was a Förster resonance energy transfer(FRET)cas-sette with a 92.8% efficiency.The large two-photon absorption cross-section of TQ allowed photosensitizer TQ-NBS to work in a 900nm two-photon excitation(TPE)mode,which greatly benefited the deep tissue penetration in PDT treatment.Meanwhile,the excellent phototoxicity and near-infrared fluorescence of NBS-NH2 was kept in TQ-NBS under a TPE mode via a FRET process.Photosensitizer TQ-NBS exhibited a high phototoxic efficacy in living cells and tumor-bearing mice.
基金项目
National Key Research and Development Program of China(2022YFA1207600)
National Natural Science Foundation of China(22178395)
National Natural Science Foundation of China(62175262)
National Natural Science Foundation of China(62005294)
维普
万方数据