Genome mining of multi-substituted alkylresorcinols from a hybrid highly reducing-and type Ⅲ-polyketide pathway

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外文摘要：Fungal alkylresorcinols are a class of polyketides,which are commonly synthesized by the hybridization of highly reducing polyketide synthase(hrPKS)with non-reducing polyketide synthase(nrPKS).In this study,we identified and demonstrated a new assembly model for synthesizing alkylresorcinol(scirpilin A,1),which was accomplished by collaboration of a hrPKS(FscA)and a type Ⅲ PKS(FscB).Furthermore,three post-tailoring enzymes(FscC,FscD,and FscE)act iteratively on 1 skeleton,including successive 14e-oxidation of inert carbons,di-halogenation,and di-methylation,to form highly oxidized and multi-substituted alkylresorcinols.Our work presents an unusual synthesis manner of alkylresorcinols,sheds light on the collaborative mechanism between hrPKS and type Ⅲ PKS and provides three valuable enzy-matic catalysts for the tailoring of alkylresorcinol family natural products in future.

外文关键词：

Genome miningAlkylresorcinolhrPKSType Ⅲ PKSIterative catalysis

作者：

Huaran Zhang、Yuting Huang、Yingjie Tang、Dekun Kong、Yi Zou

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作者单位：

College of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China

基金：

National Natural Science Foundation of ChinaNational Key R&D Program of ChinaFundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central UniversitiesScience and Technology Innovation Key R&D Program of ChongqingChongqing Science Funds for Distinguished Young Scientists

项目编号：

222771002020YFA0907700SWU-KQ22034SWU-XDPY22009CSTB2022TIAD-STX0015cstc2020jcyjjqX0005

出版年：

2024

DOI：

10.1016/j.cclet.2023.108968

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

年,卷(期)：2024.35(7)