Regulable toehold lock for the effective control of strand displacement reaction sequence and circuit leakage

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外文摘要：Strand displacement reaction enables the construction of enzyme-free DNA reaction networks,thus has been widely applied to DNA circuit and nanotechnology.It has the characteristics of high efficiency,uni-versality and regulatability.However,the existing regulation tools cannot enable effective control of the reaction sequence,which undoubtedly limits the construction of complex nucleic acid circuits.Herein,we developed a regulation tool,toehold lock,and achieved strict control of reaction sequence without loss of the main reaction signal output.Furthermore,we applied the tool to scenarios such as seesaw circuits,AND/OR logic gates,and entropy-driven circuits,and respectively demonstrated its significant superiority compared to the original method.We believe that the proposed toehold lock has greatly optimized the efficiency of DNA strand displacement-based networks,and we anticipate that the tool will be widely used in multiple fields.

外文关键词：

DNA circuitToehold lockDNA strand displacementReaction sequenceSeesaw circuit

作者：

Kuangdi Luo、Yang Qin、Xuehao Zhang、Hanxu Ji、Heao Zhang、Jiangtian Li、Xianjin Xiao、Xinyu Wang

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作者单位：

Department of Breast Surgery,Second Hospital of Jilin University,Changchun 130041,China

Institute of Reproductive Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

Department of Pancreatic Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China

Department of Laboratory Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

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基金：

National Key Research and Development Program of ChinaOpen Research Fund of State Key Laboratory of Bioelectronics,Southeast UniversityOpen Foundation of NHC Key Laboratory of Birth Defect for Research and Prevention(Hunan Provincial Maternal and Child HealthHunan Province Assisted Reproduction and Regenerative Medicine Clinical Demonstration Center Funded ProjectOpen Foundation of Translational Medicine National Science and Technology Infrastructure(Shanghai)

项目编号：

2021YFC2701402Sklb2021-k06KF20200072020SK4019TMSK-2021-141

出版年：

2024

DOI：

10.1016/j.cclet.2023.109104

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

年,卷(期)：2024.35(7)