Abstract
Strand displacement reaction enables the construction of enzyme-free DNA reaction networks,thus has been widely applied to DNA circuit and nanotechnology.It has the characteristics of high efficiency,uni-versality and regulatability.However,the existing regulation tools cannot enable effective control of the reaction sequence,which undoubtedly limits the construction of complex nucleic acid circuits.Herein,we developed a regulation tool,toehold lock,and achieved strict control of reaction sequence without loss of the main reaction signal output.Furthermore,we applied the tool to scenarios such as seesaw circuits,AND/OR logic gates,and entropy-driven circuits,and respectively demonstrated its significant superiority compared to the original method.We believe that the proposed toehold lock has greatly optimized the efficiency of DNA strand displacement-based networks,and we anticipate that the tool will be widely used in multiple fields.
基金项目
National Key Research and Development Program of China(2021YFC2701402)
Open Research Fund of State Key Laboratory of Bioelectronics,Southeast University(Sklb2021-k06)
Open Foundation of NHC Key Laboratory of Birth Defect for Research and Prevention(Hunan Provincial Maternal and Child Health(KF2020007)
Hunan Province Assisted Reproduction and Regenerative Medicine Clinical Demonstration Center Funded Project(2020SK4019)
Open Foundation of Translational Medicine National Science and Technology Infrastructure(Shanghai)(TMSK-2021-141)
维普
万方数据