Constructing thermoresponsive PNIPAM-based microcarriers for cell culture and enzyme-free cell harvesting

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外文摘要：The development of large-scale cell cultivation and non-invasive cell harvesting is highly desired in various fields,including biological regeneration and pharmaceutical research.When using traditional microcarriers for cell culture,trypsinization is often necessary during cell collection,leading to par-tial cells damage.In this work,we developed a thermoresponsive glass microcarrier modified with poly(γ-propargyl-ʟ-glutamate)(PPLG)and poly(N-isopropylacrylamide)(PNIPAM).We utilized these mi-crocarriers for three-dimensional cell culture and enzyme-free cell harvesting,and the results indicated that the prepared microcarriers exhibited excellent non-invasive cell culture performance.

外文关键词：

MicrocarrierTemperature-responesePNIPAMPolymersCell culture

作者：

Yunan Yuan、Zhimin Luo、Jie Chen、Chaoliang He、Kai Hao、Huayu Tian

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作者单位：

CAS Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China

School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China

Jilin Biomedical Polymers Engineering Laboratory,Changchun 130022,China

College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China

Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM),Xiamen 361005,China

Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM),Xiamen 361005

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基金：

name of National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaJilin Province Science and Technology Development Program

项目编号：

2021YFB380090051925305518732081833010519732175237316120200201075JC

出版年：

2024

DOI：

10.1016/j.cclet.2024.109549

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

年,卷(期)：2024.35(7)