Constructing thermoresponsive PNIPAM-based microcarriers for cell culture and enzyme-free cell harvesting

Yunan Yuan ¹Zhimin Luo ²Jie Chen ³Chaoliang He ⁴Kai Hao ⁵Huayu Tian⁶

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作者信息

1. CAS Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China;Jilin Biomedical Polymers Engineering Laboratory,Changchun 130022,China
2. College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM),Xiamen 361005,China
3. CAS Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;Jilin Biomedical Polymers Engineering Laboratory,Changchun 130022,China
4. CAS Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China
5. College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China
6. CAS Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China;College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;Jilin Biomedical Polymers Engineering Laboratory,Changchun 130022,China;Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM),Xiamen 361005,
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Abstract

The development of large-scale cell cultivation and non-invasive cell harvesting is highly desired in various fields,including biological regeneration and pharmaceutical research.When using traditional microcarriers for cell culture,trypsinization is often necessary during cell collection,leading to par-tial cells damage.In this work,we developed a thermoresponsive glass microcarrier modified with poly(γ-propargyl-ʟ-glutamate)(PPLG)and poly(N-isopropylacrylamide)(PNIPAM).We utilized these mi-crocarriers for three-dimensional cell culture and enzyme-free cell harvesting,and the results indicated that the prepared microcarriers exhibited excellent non-invasive cell culture performance.

Key words

Microcarrier/Temperature-responese/PNIPAM/Polymers/Cell culture

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基金项目

name of National Key Research and Development Program of China(2021YFB3800900)

National Natural Science Foundation of China(51925305)

National Natural Science Foundation of China(51873208)

National Natural Science Foundation of China(1833010)

National Natural Science Foundation of China(51973217)

National Natural Science Foundation of China(52373161)

Jilin Province Science and Technology Development Program(20200201075JC)

出版年

2024

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCDCSCD

影响因子：0.771

ISSN：1001-8417

参考文献量16

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