Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells

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外文摘要：The clustered regularly interspersed short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system is an RNA-guided platform for highly efficient and specific genome targeting in diverse organisms,which has been exploited for various applications in gene manipulation.Compared with the constantly active CRISPR/Cas9 function,conditional control of its activity can improve the performance of the system with reduced side effects and high spatiotemporal precision.The pH-responsive triplex RNA was success-ful used in CRISPR-derived RNA/trans-activating crRNA(crRNA/tracrRNA)of CRISPR/Cas9,thus affecting RNA/dead Cas9(dCas9)complex to target DNA in vitro and in vivo.This design of triplex RNA opens a new window towards the broad involvement of eukaryotic cells for conditional control of CRISPR/Cas9 function.

外文关键词：

RNA triplexpH-responsiveCRISPR/Cas9Gene manipulationEukaryotic cells

作者：

Yan Liu、Yang Wang、Jiayi Zhu、Xuxian Su、Xudong Lin、Liang Xu、Xiwen Xing

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作者单位：

Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument,School of Biomedical Engineering,Shenzhen Campus of Sun Yat-sen University,Sun Yat-sen University,Shenzhen 518107,China

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry,School of Chemistry,Sun Yat-sen University,Guangzhou 510275,China

Department of Cardiology,the first people's hospital of Foshan,Foshan 528000,China

Department of Biotechnology,College of Life Science and Technology,Jinan University,Guangzhou 510632,China

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出版年：

2024

DOI：

10.1016/j.cclet.2023.109427

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

年,卷(期)：2024.35(9)