Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver

Han-Min Wang ¹Yan-Chen Li ¹Lu-Lu Sun ²Ming-Ye Tang ³Jia Liu ⁴Jiahao Cai ⁵Lei Dong ⁶Jia Li ⁷Yi Zang ⁸Hai-Hao Han ⁹Xiao-Peng He¹⁰

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作者信息

1. Molecular Imaging Center,State Key Laboratory of Chemical Biology,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;Lingang laboratory,Shanghai 201203,China;University of Chinese Academy of Sciences,Beijing 100049,China
2. Shandong Laboratory of Yantai Drug Discovery,Bohai Rim Advanced Research Institute for Drug Discovery,Yantai 264117,China
3. School of Chinese Materia Medica,Nanjing University of Chinese Medicine,Nanjing 210023,China
4. Molecular Imaging Center,State Key Laboratory of Chemical Biology,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;School of Pharmaceutical Science and Technology,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou 310058,China
5. Lingang laboratory,Shanghai 201203,China
6. School of Pharmacy,Weifang Medical University,Weifang 261053,China
7. Molecular Imaging Center,State Key Laboratory of Chemical Biology,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;Shandong Laboratory of Yantai Drug Discovery,Bohai Rim Advanced Research Institute for Drug Discovery,Yantai 264117,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Pharmaceutical Science and Technology,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou 310058,China
8. Molecular Imaging Center,State Key Laboratory of Chemical Biology,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;Lingang laboratory,Shanghai 201203,China;University of Chinese Academy of Sciences,Beijing 100049,China;iSchool of Chinese Materia Medica,Nanjing University of Chinese Medicine,Nanjing 210023,China
9. Molecular Imaging Center,State Key Laboratory of Chemical Biology,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;Shandong Laboratory of Yantai Drug Discovery,Bohai Rim Advanced Research Institute for Drug Discovery,Yantai 264117,China;University of Chinese Academy of Sciences,Beijing 100049,China
10. Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering,Feringa Nobel Prize Scientist Joint Research Center,Frontiers Center for Materiobiology and Dynamic Chemistry,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China;The International Cooperation Laboratory on Signal Transduction,National Center for Liver Cancer,Eastern Hepatobiliary Surgery Hospital,Shanghai 200438,Chi
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Abstract

Non-alcoholic fatty liver disease(NAFLD)is prevalent worldwide as a chronic liver disease that not only gives rise to hepatic complications,but leads to other chronic diseases such as type 2 diabetes and atherosclerosis.The aberrant accumulation of lipid droplets(LDs)in hepatocytes is a prominent signa-ture of NAFLD.However,conventional techniques lack the capability to effectively monitor the dynamic changes in LD levels during NAFLD with living organisms.Hence,it is imperative to develop LD-specific long-wavelength fluorescent probes with high imaging contrast for the in-situ diagnosis of NAFLD.In this study,we synthesized a new LD-selective long-wavelength fluorescent probe,denoted as LD-1,based on the twisted intramolecular charge transfer(TICT)mechanism.The probe exhibits a large Stokes shift and intensive fluorescence emission in nonpolar and viscous solutions.By self-assembling LD-1 with bovine serum albumin(BSA),a biocompatible,long-wavelength fluorescent probe hybrid,LD-1@BSA,was formed,allowing for LDs to be selectively imaged in hepatocytes.Moreover,LD-1@BSA successfully dis-criminates NAFLD cells before and after drug treatment,and achieves non-invasive and real-time moni-toring of LD accumulation in a mouse model of NAFLD.

Key words

Lipid droplets imaging/Fluorescent probe/Large Stokes shift/Protein encapsulation/Non-alcoholic fatty liver

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出版年

2024

中国化学快报(英文版)

中国化学会

中国化学快报(英文版)

CSTPCD

影响因子：0.771

ISSN：1001-8417

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