Objective To investigate the expression ofheme oxygenase-1 (HO-1) by high glucose treatment in human lung epithelial A549 cells.Methods The effect of high glucose on the expression of HO-1 in A549 cells was investigated with Western blot and reverse transcription PCR.HO-1 enzymic activity and reactive oxygen species (ROS) production were investigated with enzyme-linked immunosorbent test.Results Treatment with 25 mmol/L high glucose for 0,24 h,48 h,72 h and in concentrations of 5 mmol/L,10 mmol/L,25 mmol/L,40 mmol/L for 48 h induced increased expression on protein and mRNA level of HO-1 in a concentration-and time-dependent manner in A549 cells.High glucose treatment increased production of ROS and transforming growth factor-β1 (TGF-β1) in A549 cells,which thus mediated HO-1 expression.Following the increase in HO-1 expression,the enzymatic activity of rO-1 also increased in high glucose-treated cells.Pretreatment with N-acetyl-L-cysteine (NAC) and PI3K/Akt inhibitors attenuated the high glucose-induced increased HO-1 expression.Conclusions High glucose increases ROS and TGF-β1 production in A549 cells,which mediates HO-1 expression and increases HO-1 enzymic activity.
Heme oxygenase-1Human lung epithelial cellsHigh glucoseReactive oxygen species
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