Serum lncRNA MIAT and miR-515-5p expression levels and clinical significance in elderly patients with chronic obstructive pulmonary disease at different periods
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
目的 探究老年慢性阻塞性肺疾病(慢阻肺)患者不同时期血清长链非编码RNA心肌梗死相关转录本(long noncoding RNA myocardial infarction associated transcript,IncRNA MIAT)、微小 RNA-515-5p(microRNA-515-5p,miR-515-5p)的表达水平及临床意义.方法 选取2021年4月-2023年6月在淮北市人民医院就诊的老年慢阻肺急性加重期患者90例作为慢阻肺急性加重组,老年慢阻肺稳定期患者88例作为慢阻肺稳定组,以及体检健康老年人90例作为对照组.检测所有受试者患者白细胞计数(white blood cell count,WBC)和血清lncRNA MIAT和miR-515-5p表达水平,慢阻肺患者血气分析及肺功能指标[氧合指数(PaO2/FiO2)、动脉血二氧化碳分压(PaCO2)、第1秒用力呼气容积与用力肺活量的比值(forced expiratory volume in the first second/forced vital capacity,FEV1/FVC)、第 1 秒用力呼气容积占预计值百分比(FEV1 as a percentage of predicted value,FEV1％pred)].分析老年慢阻肺急性加重期患者血清lncRNA MIAT、miR-515-5p与吸烟、WBC、血气分析及肺功能指标间的相关性,老年慢阻肺患者急性加重发生的影响因素,以及血清lncRNA MIAT、miR-515-5p预测老年慢阻肺患者急性加重发生的价值.结果 对照组、慢阻肺稳定组、慢阻肺急性加重组吸烟比例、WBC、血清lncRNA MIAT表达水平依次升高,血清miR-515-5p表达水平依次降低(P＜0.05).与慢阻肺稳定组比较,慢阻肺急性加重组PaCO2显著升高,PaO2/FiO2、FEV1/FVC、FEV1％pred显著降低(P＜0.05).老年慢阻肺急性加重期患者血清 lncRNA MIAT 与吸烟、WBC、PaCO2 呈正相关(P＜0.05),与 PaO2/FiO2、FEV1/FVC、FEV1％pred、miR-515-5p呈负相关(P＜0.05);血清 miR-515-5p 与吸烟、WBC、PaCO2 呈负相关(P＜0.05),与 PaO2/FiO2、FEV1/FVC、FEV1％pred呈正相关(P＜0.05).吸烟、WBC、PaCO2、lncRNA MIAT是影响老年慢阻肺患者急性加重发生的危险因素,PaO2/FiO2、FEV1/FVC、FEV1％pred、miR-515-5p是影响老年慢阻肺患者急性加重发生的保护因素(P＜0.05).血清lncRNA MIAT、miR-515-5p以及两者联合预测老年慢阻肺患者急性加重发生的受试者操作特征曲线下面积(area under ROC curve,AUC)分别为 0.823、0.862、0.919,高于血清lncRNA MIAT、miR-515-5p 各自单独预测的AUC(P＜0.05).结论 老年慢阻肺患者血清lncRNA MIAT呈高表达,血清miR-515-5p呈低表达,且急性加重期患者二者变化更明显.二者与急性加重期患者血气分析指标及肺功能相关,对预测老年慢阻肺患者急性加重发生有较高价值.
Objective To explore the expression levels and clinical significance of serum long noncoding RNA myocardial infarction associated transcript(lncRNA MIAT)and microRNA-515-5p(miR-515-5p)in elderly patients with chronic obstructive pulmonary disease(COPD)at different periods.Methods From April 2021 to June 2023,90 elderly patients with acute exacerbation of COPD treated in Huaibei People's Hospital were selected as a COPD acute exacerbation group,88 elderly patients with stable COPD as a COPD stable group,and 90 healthy elderly individuals undergoing physical examination as a control group.The white blood cell count(WBC)and serum lncRNA MIAT and miR-515-5p expression levels were detected in all subjects,blood gas analysis and pulmonary function indexes[oxygenation index(PaO2/FiO2),arterial blood carbon dioxide partial pressure(PaCO2),ratio of forced expiratory volume in the first second to forced vital capacity(FEV1/FVC),and FEV,as a percentage of predicted value(FEV1％pred)]were detected in the patients with COPD.The correlation between serum lncRNA MIAT,miR-515-5p and smoking,WBC,blood gas analysis and pulmonary function indexes were analyzed in the elderly patients with acute exacerbation of COPD.The influencing factors of acute exacerbation of COPD,and the value of serum lncRNA MIAT,miR-515-5p in predicting the occurrence of acute exacerbation of COPD were also analyzed.Results The smoking proportion,WBC,serum lncRNA MIAT expression levels of the control group,the COPD stable group and the COPD acute exacerbation group were increased in turn,serum miR-515-5p expression levels were decreased in turn(P＜0.05).Compared with the COPD stable group,PaCO2 was significantly increased in the COPD acute exacerbation group,while PaO2/FiO2,FEV1/FVC and FEV1％pred were significantly decreased(P＜0.05);serum lncRNA MIAT in the elderly patients with acute exacerbation of COPD was positively correlated with smoking,WBC,PaCO2(P＜0.05),and negatively correlated with PaO2/FiO2,FEV1/FVC,FEV1％pred,miR-515-5p(P＜0.05);serum miR-515-5p was negatively correlated with smoking,WBC,PaCO2(P＜0.05),and positively correlated with PaO2/FiO2,FEV1/FVC,FEV1％pred(P＜0.05).Smoking,WBC,PaCO2,and lncRNA MIAT were risk factors affecting the acute exacerbation of COPD patients,PaO2/FiO2,FEV1/FVC,FEV1％pred,miR-515-5p were protective factors affecting the acute exacerbation of elderly COPD patients(P＜0.05).The area under the ROC curve(AUC)of serum lncRNA MIAT,miR-515-5p and their combination in predicting acute exacerbation in elderly COPD patients were 0.823,0.862 and 0.919,respectively,higher than the AUC predicted by serum lncRNA MIAT and miR-515-5p separately(P＜0.05).Conclusions Serum lncRNA MIAT expression was high in elderly patients with COPD,and serum miR-515-5p expression was low,and the changes of both were more obvious in patients with acute exacerbation.Both were correlated with blood gas analysis and pulmonary function indexes in patients with acute exacerbation,and have high value in predicting the occurrence of acute exacerbation in elderly patients with COPD.
NETL
NSTL
万方数据
