Secondary metabolites and their bioactivities of a soft coral-derived fungus Aspergillus versicolor (ZJ-2008015)
Objective To investigate the secondary metabolites and their bioactivities of a soft coral-derived fungus. Methods Under the guidance of bioassay, column chromatography on silica gel and Sepha-dex LH-20, and semi-preparative HPLC were used to isolate and purify the metabolites from the fermentation products of Aspergillus versicolor (ZJ-2008015) , a fungus obtained from a soft coral Sarco-phyton sp. collected from the South China Sea. The structures of the isolated compounds were identified by spectroscopic analysis including NMR and MS, and by comparison with the data of literature. The bioactivities of the compounds were evaluated with brine shrimp lethality and antibacteria models.Results Six compounds were isolated from the EtOAc extracts of the culture broth and the mycelia of A.versicolor (ZJ-2008015), and their structures were identified as (+)-sydonic acid (1), expansol G (2),(+)-sydowic acid (3), cyclo (L-Trp-L-Phe) (4), homodimeric WIN 64821 (5), and ergosta-6α-rhy-droxy-4,22-dien-3-one (6). Conclusion The bisabolane sesquiterpene compounds 1-3 exhibited potentantibacterial activity against Staphylococcus albus and Staphylococcus aureus.
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