A new naphthoquinone derivative isolated from Penicillium raistrickii
Objective To explore the secondary metabolites chemical diversity of the talent fungus Penicillium raistrickii derived from saline soil based on the strategy of one strain,many compounds(OSMAC),and to evaluate the proliferation inhibition effect of the secondary metabolites towards HepG2 cell lines.Methods Rice was applied as the culture medium for the fungus with incubation period of 30 days at static station,followed by extraction with ethyl acetate three times to get the crude extract.The isolation and purification process were carried out with silica gel column chromatography,LH20 column chromatography,semi-preparative HPLC or semi-preparative TLC techniques.The structures of the isolated compounds were elucidated by extensive NMR and HR-ESI-MS analyses,as well as comparisons with the data reported.The proliferation inhibition effects of the isolated compounds against HepG2 cell line were evaluated by CCK8 method.Results Ten polyketides,including two naphthoquinone derivatives(1-2),four chromones(3-6)and four xanthones(7-10)were obtained and identified as 7-carbomethoxy-2,8-dimethoxy-5-hydroxy-1,4-naphthoquinone(1),2,7-dimethoxy-5-hydroxy-1,4-naphthoquinone(2),2-ethyl-7-hydroxy-5-methyl chromone(3),2,5-dimethyl-7-hydroxy chromone(4),5-carboxymethyl-7-hydroxy-2-methyl chromone(5),lamellicolic anhydride(6),1,3,6-trihydroxy-8-methyl xanthone(7),1,6-dihydroxy-3-methoxy-8-methyl xanthone(8),1,5,6-trihydroxy-3-methoxy-8-methyl xanthone(9)and 1,3,5,6-tetrhydroxy-8-methyl xanthone(10).Conclusion The chemical diversity of P.raistrickii was investigated based OSMAC strategy,and ten polyketides were isolated and identified.Among them,compound 1 was a new naphthoquinone derivative and compound 3 was a new natural chromone product.None of the isolated compounds exhibited proliferation inhibition effect towards HepG2 cell line.
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