Study on the anti-inflammatory effect of brain glycosides from marine symbiotic bacteria on RAW264.7 cell
Marine microorganisms characterized by rapid growth,adjustable metabolism,easy breeding and sustainable utilization become a new source of pharmacological active substances.In this study,brain glycosides were extracted from marine symbiotic bacteria.CCK8 assay was used to detect cell activity of RAW264.7.After the establishment of cell inflammation model,the contents of inflammatory factors IL-6 and IL-1β in brain glycosides were detected,and the mRNA levels of IL-6,IL-1β,TNF-α,McP-1,MIP-2 and INOS were detected by fluorescence quantitative PCR.Meanwhile,the expression levels of inflammatory signaling pathway proteins p-ERK1/2,MyD88,NF-κB,NLRP3,p62,p-IKB and p-JNK were detected by Western blotting.The results showed that brain glycosides could decrease the contents and mRNA levels of inflammatory factors IL-6,IL-1β,TNF-α,MCP-1,MIP-2 and INOS,and inhibit the protein expression levels of p-ERK1/2,MyD88,NF-κB,NLRP3,p-IKB and p-JNK.These results suggested that the anti-inflammatory effect of brain glycosides on lipopolysaccharide induced RAW264.7 cells was realized by regulating the MyD88/NF-κB/MAPK/NLRP3 signaling pathway.This study provided a reference for the use of brain glycosides in the treatment of inflammation-related diseases and laid a foundation for the development of new marine drugs.
万方数据
维普
