Objective To identify the species and study the secondary metabolites from a fungus Alternaria alternata GXIMD 02507,which was derived from the coral Pocillopora damicornis collected from the Weizhou island.Methods The species of the strain were identified by ITS sequence analysis.The secondary metabolites were separated and purified by various chromatographic methods exemplified by silica gel column chromatography and semi-high-performance liquid chromatography,and their structures were determined by analysis of NMR and CD data as well as by comparison with literature data.Results A total of eight compounds,predominantly belonging to altenuene deravitives,were obtained from its solid rice fermented products,which were determined as altenuene(1),4'-epialtenuene(2),dihydroaltenuene A(3),alternariol(4),saponaroxin A(5),zinniol(6),2-(4-hydroxyphenyl)ethyl acetate(7),and lumichrome(8),respectively.Conclusion Four altenuene deravitives,two phenolics,one tricyclic polyketide,and one lumichrome were characterized from this fungus.Meanwhile,compounds 1-7 exhibited significant inhibition of lipopolysaccharide(LPS)-induced NF-κB activation in RAW 264.7 macrophages at 20 μmol/L.
关键词
北部湾/造礁珊瑚/链格孢菌/代谢产物/NF-κB
Key words
the Beibu Gulf/scleractinian corals/Alternaria alternata/secondary metabolites/NF-κB
维普
万方数据