Study on secondary metabolites and insecticidal activities of the fungus Fusariumsolani sp.from Kandelia candela
Objective To study the secondary metabolites and insecticidal activities of the fungus Fusariumsolani sp.QJS4-1-2 derived from a medicinal mangrove Kandelia candela.And grow inhibitions of these compounds to Helicoverpa armigera larvae were also studied.Methods The ethyl acetate crude extracted from the solid fermentation product of fungus QJS4-1-2 was used to separate bioactive compounds.Normal and reverse phase silica gel,Sephadex LH-20,semi-preparative HPLC column chromatography and thin layer chromatography were used to isolate compounds.And then their structures were identified by 1D NMR,MS,together with other spectral analysis methods and comparison with literature data.All of these compounds were tested for the insecticidal activity against Helicoverpa armigera.Results Ten known compounds were isolated from the fungus Fusariumsolani sp.QJS4-1-2,including three coumarins(1-3),two isocoumarins(4,5),three xanthones(6-8)and two benzene derivatives(9,10).They structures were identified as orlandin(1),demethylkotanin(2),7-hydroxy-4-methoxy-5-methylcoumarin(3),3S,4R-(+)-4-hydroxymellein(4),6-hydroxy-8-methoxy-3-methyl-1H-2-benzopyran-1-one(5),rubrofusarin B(6),5-hydroxy-2-hydroxymethyl-4H-chromen-4-one(7),flavasperone(8),2-[(R)-hydroxyphenyl-methyl]-4H-pyran-4-one(9),and aspernigrin A(10).Compound 9 showed potent inhibitory activity against Helicoverpa armigera with the IC50 value of 100 μg/mL.Conclusion Compounds 1-10 were isolated from the endophytic fungi of Kandelia candela,and one compound had growth inhibition activity against larvae of Helicoverpa armigera.These results provided a basis for the development and utilization of Kandelia candela.
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