Studies on diterpenoids of soft coral Lobophytum sp.from South China Sea
Objective To investigate the structural diversity and biological activity of chemical constituents from soft coral lobophytum sp..Methods The compounds were isolated from crude extract and purified by silica gel column,ODS-C18 column,Sephadex LH-20 and HPLC.Their structures were determined by analysis of UV,NMR,MS data and comparison with those reported in literatures.Results Ten cembrane diterpenoids were isolated and identified as sarcomililatin B(1),lobophytin A(2),(-)-isosarcophine(3),(+)-sarcophytoxide(4),isosarcophytoxide(5),(+)-11,12-epoxy-11,12-dihydrocembrene-C(6),cherbonnolide B(7),sarcomililatins A(8),sarcophytonin B(9)and 3,4-dihydro-4α-hydroxy-Δ2-sarcophine(10).Compounds 7 and 8 were isolated from this genus for the first time.At a concentration of 20 μmol/L,compounds 1 and 3 exhibited inhibition of LPS-induced NO production with inhibitory rates as 31.5％and 28.6％,respectively.Conclusion Ten cembrane diterpenoids were isolated from soft corals Lobophytum sp..Compounds 1 and 3 showed inhibition effects on NO production induced by LPS.
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