摘要
目的 对海洋来源软珊瑚Lobophytum sp.中的化学成分结构多样性及生物活性进行研究.方法 利用硅胶、十八烷基硅烷键合硅胶(ODS-C18)、凝胶Sephadex LH-20和半制备高效液相(semi-HPLC)等色谱学方法对软珊瑚乙酸乙酯提取物中的化学成分进行分离;利用紫外(UV)、核磁共振波谱(NMR)、质谱(MS)等波谱学方法,结合与文献的数据对比确定化合物的结构.结果 从软珊瑚的乙酸乙酯提取物中分离鉴定了 10个西松烷型二萜类化合物,分别为 sarcomililatin B(1)、lobophytin A(2)、(-)-isosarcophine(3)、(+)-sarcophytoxide(4)、isosarcophytoxide(5)、(+)-11,12-epoxy-11,12-dihydrocembrene-C(6)、cherbonnolide B(7)、sarcomililatins A(8)、sarcophytonin B(9)和 3,4-dihydro-4α-hydroxy-Δ2-sarcophine(10).其中化合物 7 和 8 为首次从该属软珊瑚中分离得到.化合物1和3在20 μmol/L物质的量浓度下对脂多糖(LPS)诱导NO产生的抑制率分别为31.5％和28.6％.结论 从南海软珊瑚Lobophytum sp.中分离得到了化合物1～10,其中化合物1和3对LPS诱导NO产生具有一定的抑制作用.
Abstract
Objective To investigate the structural diversity and biological activity of chemical constituents from soft coral lobophytum sp..Methods The compounds were isolated from crude extract and purified by silica gel column,ODS-C18 column,Sephadex LH-20 and HPLC.Their structures were determined by analysis of UV,NMR,MS data and comparison with those reported in literatures.Results Ten cembrane diterpenoids were isolated and identified as sarcomililatin B(1),lobophytin A(2),(-)-isosarcophine(3),(+)-sarcophytoxide(4),isosarcophytoxide(5),(+)-11,12-epoxy-11,12-dihydrocembrene-C(6),cherbonnolide B(7),sarcomililatins A(8),sarcophytonin B(9)and 3,4-dihydro-4α-hydroxy-Δ2-sarcophine(10).Compounds 7 and 8 were isolated from this genus for the first time.At a concentration of 20 μmol/L,compounds 1 and 3 exhibited inhibition of LPS-induced NO production with inhibitory rates as 31.5％and 28.6％,respectively.Conclusion Ten cembrane diterpenoids were isolated from soft corals Lobophytum sp..Compounds 1 and 3 showed inhibition effects on NO production induced by LPS.
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