Study on secondary metabolites from Antarctic-intertidal derived fungi Pseudeurotium sp.HDN17-894
Objective To study secondary metabolites from fungi Pseudeurotium sp.HDN17-894 derived from the intertidal zone of Antarctic and their antimicrobial activities.Methods The crude extract of strain HDN 17-894 was obtained by large-scale fermentation.Silica gel column chromatography,semi-preparative HPLC and Sephadex LH-20 were used to separate and purify the crude extract.By comparison with data of relevant literatures,the structures of the compounds were determined by NMR,MS and single crystal diffraction.The antimicrobial compounds were screened in vitro.Results A pair of new indol-3-one spiro racemic compounds(±)-pseudeurdole(1),two known sulochrin compounds(2-3),three known diphenyl ethers(4-6)and one known polyketone compound(7)were isolated from the fungus HDN17-894.(±)-pseudeurdole(1)were compounds containing indole-3-one spirohexadienone skeleton from natural products for the first time.The antimicrobial activity of these compounds was evaluated,and the results revealed that compounds 3 and 7 showed antimicrobial potential against different pathogenic bacteria.Among them,the MIC value of compound 3 against B.cereus was 16 μg/mL.