Studies on secondary metabolites and activities of the fungus Fusarium oxysporum RD-79 from the Yellow River Delta
Objective To study the secondary metabolites and their biological activities of a fungus Fusarium oxysporum RD-79 from the coastal wetland of the Yellow River Delta.Methods The fermentation products of the strain were separated and purified by silica gel column chromatography,ODS column chromatography,semi-preparative high performance liquid chromatography and other separation methods.Their structures were elucidated based on the spectral data of nuclear magnetic resonance analyses(NMR)and mass spectrometry(MS)and compared with literature data.CCK8 and Griess methods were used to test the cytotoxic activity and anti-inflammatory activity of the compounds.Results Seven pure compounds were isolated and identified as 5-butyl-4-methoxy-6-methyl-2H-pyran-2-one(1),delitpyrone D(2),3-hydroxymethyl-6,8-dimethoxycoumaarin(3),5-[(1S,2S)-1,2-dihydroxybutyl]-4-methoxy-6-methyl-2H-pyran-2-one(4),setosphapyrone A(5),5-(1-hydroxybutyl)-4-methoxy-6-methyl-2H-pyran-2-one(6),9-O-methylscytalol A(7),respectively.Conclusion Compounds 1-7 could inhibit the production of NO in varying degrees.At the concentration of 100 μg/mL,compounds 1-7 showed no anti-tumor activity.
Yellow River Deltafungussecondary metabolitesNO inhibitory activity
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