黄河三角洲来源真菌Fusarium oxysporum RD-79次级代谢产物研究
Studies on secondary metabolites and activities of the fungus Fusarium oxysporum RD-79 from the Yellow River Delta
胡晓燕 1苟春霞 1卢立明 1苗双 2宫凯凯2
作者信息
- 1. 滨州医学院附属医院药学部,山东滨州 256603
- 2. 滨州医学院附属医院医学研究中心,山东滨州 256603
- 折叠
摘要
目的 研究1株黄河三角洲滨海湿地来源的真菌Fusarium oxysporum RD-79的次级代谢产物及其生物活性.方法 利用硅胶柱色谱、凝胶柱色谱、半制备高效液相色谱等分离方法对该菌株发酵产物进行分离纯化,通过核磁共振(NMR)、质谱(MS)等方法,结合相关文献对比鉴定化合物结构;分别采用CCK8法和Griess法对化合物进行抗肿瘤活性和抗炎活性测试.结果 从Fusarium oxysporum RD-79的发酵产物中分离得到7个单体化合物,分别为 5-butyl-4-methoxy-6-methyl-2H-pyran-2-one(1)、delitpyrone D(2)、3-hydroxymethyl-6,8-dimethoxycoumaarin(3)、5-[(1S,2S)-1,2-dihydroxybutyl]-4-methoxy-6-methyl-2H-pyran-2-one(4)、setosp-hapyrone A(5)、5-(1-hydroxybutyl)-4-methoxy-6-methyl-2H-pyran-2-one(6)、9-O-methylscytalol A(7).结论 化合物1～7均可不同程度抑制NO的产生;在质量浓度为100 μg/mL下,化合物1～7均无抗肿瘤活性.
Abstract
Objective To study the secondary metabolites and their biological activities of a fungus Fusarium oxysporum RD-79 from the coastal wetland of the Yellow River Delta.Methods The fermentation products of the strain were separated and purified by silica gel column chromatography,ODS column chromatography,semi-preparative high performance liquid chromatography and other separation methods.Their structures were elucidated based on the spectral data of nuclear magnetic resonance analyses(NMR)and mass spectrometry(MS)and compared with literature data.CCK8 and Griess methods were used to test the cytotoxic activity and anti-inflammatory activity of the compounds.Results Seven pure compounds were isolated and identified as 5-butyl-4-methoxy-6-methyl-2H-pyran-2-one(1),delitpyrone D(2),3-hydroxymethyl-6,8-dimethoxycoumaarin(3),5-[(1S,2S)-1,2-dihydroxybutyl]-4-methoxy-6-methyl-2H-pyran-2-one(4),setosphapyrone A(5),5-(1-hydroxybutyl)-4-methoxy-6-methyl-2H-pyran-2-one(6),9-O-methylscytalol A(7),respectively.Conclusion Compounds 1-7 could inhibit the production of NO in varying degrees.At the concentration of 100 μg/mL,compounds 1-7 showed no anti-tumor activity.
关键词
黄河三角洲/真菌/次级代谢产物/一氧化氮抑制活性Key words
Yellow River Delta/fungus/secondary metabolites/NO inhibitory activity引用本文复制引用
基金项目
国家自然科学基金(81903537)
山东省自然科学基金(ZR2018BB024)
山东省中医药科技发展计划(2019-0521)
滨州医学院科技项目(BY2017KJ17)
出版年
2024
NETL
NSTL
万方数据