Preparation of β-glucans from six kinds of brown algae and studies on their structures and immunological activities
Objective To investigate the structure and immunological activity of β-glucan from six kinds of brown algae,and provide the algae source for the development of marine carbohydrate-based drugs.Methodsβ-glucans were prepared from 6 kinds of brown algae by hot water extraction,ethanol precipitation and column chromatography.Their structures were characterized by high performance liquid chromatography(HPLC),infrared radiation(IR)and nuclear magnetic resonance spectroscopy(1H NMR,13C NMR and 1H 13C HSQC),and immune activities were evaluated by phagocytosis of neutral red and NO release amount of mouse macrophage RAW264.7.Results In this paper,six kinds of β-glucans with different structures were prepared,and their structures were analyzed.It was proved that they contained β-1,3 and β-1,6 linkages,and their relative molecular weights were 2 290～5 490,Durvillaea antarctica,which has the highest content of β-glucan and the best immune activity,is an ideal resource for β-glucan drug development.
brown algaeβ-glucanstructural charactersimmune activity
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