6种褐藻β-葡聚糖的制备及其结构与免疫活性研究
Preparation of β-glucans from six kinds of brown algae and studies on their structures and immunological activities
张传振 1吕友晶 2王春蕾 3李全才 2胡婷 2赵峡 2李建杰 3蔡超 1于广利2
作者信息
- 1. 中国海洋大学海洋药物教育部重点实验室,山东省糖科学与糖工程重点实验室,医药学院,山东青岛 266003
- 2. 中国海洋大学海洋药物教育部重点实验室,山东省糖科学与糖工程重点实验室,医药学院,山东青岛 266003;青岛海洋生物医药研究院,山东青岛 266100
- 3. 青岛海洋生物医药研究院,山东青岛 266100
- 折叠
摘要
目的 研究不同褐藻来源β-葡聚糖的结构及免疫活性差异,为β-葡聚糖药物的开发提供合适的藻源.方法 通过热水提取、乙醇沉淀和柱层析分离从6种褐藻中制备β-葡聚糖,并利用高效液相色谱(HPLC)、红外光谱(IR)、核磁共振波谱(1H NMR,13C NMR和1H 13C HSQC)对其结构进行表征.通过小鼠巨噬细胞RAW264.7吞噬中性红及NO释放实验评价其免疫调节活性.结果 本文制备了6种结构不同的β-葡聚糖,对其结构分析,证明其含有β-1,3和β-1,6连接,相对分子质量为2 290～5 490,海茸中β-葡聚糖的含量最高且具有最优的免疫活性,是β-葡聚糖药物开发的良好资源.
Abstract
Objective To investigate the structure and immunological activity of β-glucan from six kinds of brown algae,and provide the algae source for the development of marine carbohydrate-based drugs.Methodsβ-glucans were prepared from 6 kinds of brown algae by hot water extraction,ethanol precipitation and column chromatography.Their structures were characterized by high performance liquid chromatography(HPLC),infrared radiation(IR)and nuclear magnetic resonance spectroscopy(1H NMR,13C NMR and 1H 13C HSQC),and immune activities were evaluated by phagocytosis of neutral red and NO release amount of mouse macrophage RAW264.7.Results In this paper,six kinds of β-glucans with different structures were prepared,and their structures were analyzed.It was proved that they contained β-1,3 and β-1,6 linkages,and their relative molecular weights were 2 290～5 490,Durvillaea antarctica,which has the highest content of β-glucan and the best immune activity,is an ideal resource for β-glucan drug development.
关键词
褐藻/β-葡聚糖/结构特点/免疫活性Key words
brown algae/β-glucan/structural characters/immune activity引用本文复制引用
基金项目
山东省科技攻关重点项目(2021ZDSYS22)
国家基金委重大基础研究项目(81991522)
国家基金委联合基金重点项目(U21A20297)
山东省泰山学者攀登计划(TSPD20210304)
出版年
2024
NETL
NSTL
万方数据