首页|乌贼墨纳米颗粒制备工艺优化及肿瘤光热治疗应用研究

乌贼墨纳米颗粒制备工艺优化及肿瘤光热治疗应用研究

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目的 分离纯化乌贼墨纳米颗粒(squid ink nanoparticles,SINPs),开展材料学表征,考察其肿瘤光热治疗(photothermal therapy,PTT)作用.方法 通过搅拌、超声和离心纯化出SINPs,在近红外激光(near infra-red,NIR)照射下考察其体外光热转化效果,采用CCK-8法测定细胞存活率,通过对小鼠瘤内注射给药的同时给予激光照射,研究其体内抑瘤效果.结果 制备的SINPs粒径为(212±1.4)nm,Zeta电位为(-14.5±0.7)mV.测定其光热转化效率为35.5%,说明其可作为1种光热转化剂.800 μg/mLSINPs在808 nm激光照射下小鼠乳腺癌细胞(4T1)的存活率为67.3%,表明SINPs联合PTT对4T1细胞有一定的杀伤作用,此外,在荷瘤小鼠体内,空白组瘤质量为SINPs高+laser组瘤质量的2.3倍,说明SINPs联合PTT有一定的抗瘤作用.结论 SINPs联合PTT能显著抑制荷瘤小鼠体内肿瘤组织的生长.作为1种天然纳米材料,SINPs具有良好的肿瘤PTT作用,提示其除传统药理作用外,作为天然、安全的纳米材料或载体,在肿瘤光学治疗领域具有一定应用前景.
Study on preparation optimization and application of tumor photothermal therapy of squid ink nanoparticles
Objective Squid ink nanoparticles(SINPs)were isolated and enriched to characterize the nanoparticles and investigate the role of photothermal therapy(PTT).Methods SINPs were purified by stirring,ultrasound and centrifugation,and the photothermal transformation effect in vitro was investigated under the irradiation of near infra-red(NIR).The cell viability was determined by CCK-8 method,and the tumor inhibitory effect in vivo was studied by laser irradiation combined with intratumoral injection in mice.Results The particle size of SINPs was(212±1.4)nm and Zeta potential was(-14.5±0.7)mV.The photothermal conversion efficiency was 35.5%,indicating that it could be used as a photothermal agents.The cell viability of mouse breast cancer cells(4T1)was 67.3%under 808 nm laser irradiation with 800 μg/mL SINPs,indicating that SINPs combined with PTT had certain killing effect to 4T1 cells.In addition,the tumor weight of the control group was 2.3 times that of the SINPs+laser group.It indicated that SINPs combined with PTT had certain antitumor effect.Conclusion SINPs combined with PTT could significantly inhibit the growth of tumor tissue in tumor bearing mice.As a natural nanomaterial,SINPs has a good PTT effect on tumor,suggesting that in addition to traditional pharmacological effects,SINPs as a natural and safe nanomaterial has a certain application prospect in the field of tumor optical therapy.

squidsquid ink nanoparticlesphotothermal conversion efficiencyphotothermal treatment of tumor

刘澳莹、张鹏、杨文哲、卢乾、杨雪、潘雪

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中国海洋大学医药学院,山东青岛 266003

青岛海洋生物医药研究院股份有限公司,山东青岛 266071

乌贼 乌贼墨纳米颗粒 光热转化效率 肿瘤光热治疗

国家自然科学基金项目中央引导地方科技发展专项

81901843YDZX2021018

2024

中国海洋药物
中国药学会

中国海洋药物

CSTPCD
影响因子:0.539
ISSN:1002-3461
年,卷(期):2024.43(3)