Study on preparation optimization and application of tumor photothermal therapy of squid ink nanoparticles
Objective Squid ink nanoparticles(SINPs)were isolated and enriched to characterize the nanoparticles and investigate the role of photothermal therapy(PTT).Methods SINPs were purified by stirring,ultrasound and centrifugation,and the photothermal transformation effect in vitro was investigated under the irradiation of near infra-red(NIR).The cell viability was determined by CCK-8 method,and the tumor inhibitory effect in vivo was studied by laser irradiation combined with intratumoral injection in mice.Results The particle size of SINPs was(212±1.4)nm and Zeta potential was(-14.5±0.7)mV.The photothermal conversion efficiency was 35.5%,indicating that it could be used as a photothermal agents.The cell viability of mouse breast cancer cells(4T1)was 67.3%under 808 nm laser irradiation with 800 μg/mL SINPs,indicating that SINPs combined with PTT had certain killing effect to 4T1 cells.In addition,the tumor weight of the control group was 2.3 times that of the SINPs+laser group.It indicated that SINPs combined with PTT had certain antitumor effect.Conclusion SINPs combined with PTT could significantly inhibit the growth of tumor tissue in tumor bearing mice.As a natural nanomaterial,SINPs has a good PTT effect on tumor,suggesting that in addition to traditional pharmacological effects,SINPs as a natural and safe nanomaterial has a certain application prospect in the field of tumor optical therapy.
squidsquid ink nanoparticlesphotothermal conversion efficiencyphotothermal treatment of tumor