The secondary metabolites isolated from a mutant 3d10-01 derived from Penicillium chrysogenum S-3-25 by diethyl sulfate mutagenesis Ⅱ
Objective To investigate the secondary metabolites from diethyl sulfate(DES)mutant 3d10-01 derived from Penicillium chrysogenum S-3-25 isolated from polar deep sea fermented in artificial seawater medium.Methods The secondary metabolites were isolated by multiple separation techniques,the structures of the compounds were identified according to the physicochemical and spectral data(nuclear magnetic resonance and mass spectrometry),and the cytotoxic activities were assayed by MTT method.Results Seventeen metabolites:dehydroaustinol(1),dehydroaustin(2),austinol(3),austinolide(4),11-acetoxyisoaustinone(5),penipyridone F(6),penipyridone C(7),penipyridone D(8),l-hydroxy-3-methylxanthone(9),1,5-dihydroxy-3-methylxanthone(10),nidulalin B(11),(4S,4aR,9aR)-4a-carbomethoxy-1,4,4a,9a-tetrahydro-4,8-dihydroxy-6-methylxanthone(12),(4R,4aS,9aR)-1,9a-dihydronidulalin A(13),l-hydroxy-3-hydroxymethyl-9H-xanthen-9-one(14),1,7-dihydroxy-3-methylxanthone(15),(S)-2-methyl-3-[(Z)-benzylidene]hexahydropyrrolo[1,2-a]pyrazine-1,4-dione(16)and pestalasin A(17)were isolated and identified from its rice fermentation products.Compounds 1,3,5,6,9,10 and 15 displayed stronger cytotoxic activities against HL60 cells with the IC50 values of 1.7,15.0,16.6,26.5,2.0,6.9 and 28.4 μmol/L.Compounds 1 also displayed stronger cytotoxic activities against K562 and HeLa cells with the IC50 values of 24.2 and 25.4 μmol/L.Conclusion Seventeen compounds were isolated from diethyl sulfate(DES)mutant 3d10-01 derived from the fungus Penicillium chrysogenum S-3-25.Compound 10 and 16 were new natural products and had never been reported in the literature.Compounds 1,3,5,6,9,10 and 15 displayed stronger cytotoxic activities against HL60 cells and compounds 1 also displayed stronger cytotoxic activities against K562 and HeLa cells.
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