Secondary metabolites of South China Sea cold seep sediment-derived fungus Penicillium guanacastense HDN20-0005
Objective To study the secondary metabolites of South China Sea cold seep sediment-derived fungus Penicillium guanacastense HDN20-0005.Methods The culture conditions of the strain were optimized,and the fermented products were isolated and purified by silica gel column chromatography,ODS reverse phase column chromatography,and semi-preparative high-performance liquid chromatography(HPLC).The structures of compounds were analyzed by mass spectrometry(MS),nuclear magnetic resonance spectroscopy(NMR),calculation of ECD,DP4+analysis,and comparison with reported literature.The antimicrobial activity of the compounds was evaluated.Results One new anhydride compound,(R,Z)-3-ethyl-6-methyl-3,4-dihydro-2H-1,4-oxazocine-2,5,8-trione(1),one new chromone derivative,(E)-6,7-dihydroxy-3-(3-oxobut-1-en-1-yl)-4H-chromen-4-one(2),one known chromone derivative,penialidin F(3),and one known compound,pencolide(4),were isolated from the strain.The antimicrobial activity of compounds 1-4 against seven fungi and bacteria was evaluated,and the results showed that all compounds had no antimicrobial activity.In addition,the inhibitory activity of xanthine oxidase was evaluated for the new compounds 1 and 2,and the results showed that compound 2 exhibited weak inhibitory activity against xanthine oxidase with an inhibition rate of(13.16±1.57)％.
South China Seacold seepfungussecondary metabolites
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