Research on the secondary metabolites from soft coral-associated fungus Aspergillus versicolor CGF9-1-2
Objective To study the chemical structures of secondary metabolites from soft coral derived fungus Aspergillus versicolor CGF9-1-2.Methods The secondary metabolites were separated and purified by silica gel column chromatography,octadecysilane(ODS)column chromatography,and semi-preparative HPLC.The structures of isolated compounds were subjected to high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),nuclear magnetic resonance(NMR),and optical rotation dispersion(ORD)spectroscopy.They were also tested for their DNA phosphodiesterase 1(TDP1)activities.Results From the culture of Aspergillus versicolor CGF9-1-2,we successfully isolated ten known compounds and identified as(2S,2'R,3R,3'E,4E,8E)-1-O-(β-D-glucopyranosyl)-3-hydroxyl-2-(N-2'-hydroxyl-3'-octadecenoyl)amino-9-methyl-4,8-octade-cadiene(1),(4E,8E)-N-2'-hydroxyoctade-canoyl-1-O-(β-D-glucopyranosyl)-9-methyl-4,8-sphingadienine(2),desoxybrevianamide E(3),cyclo(D-Pro-D-Phe)(4),cyclo(L-Pro-D-Leu)(5),indole-3-carboxylic-acid(6),β-carboline(7),(7S)-(+)-hydroxysydonic acid(8),ent-aspergoterpenin C(9)and(E)-7-deoxy-7,8-dide-hydro-12-hydroxysydonic acid(10).The results of TDP1 activity test showed that none of these compounds showed significant inhibitory activity.Conclusion Compound 2 was firstly isolated from Aspergillus,and com-pound 1 was obtained from Aspergillus versicolor for the first time.
marine herb soft coral-associatedAspergillus versicoloralkaloidphenolic acids
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