Discovery of alnumycins from the marine-derived Streptomyces sp.S063
Objective Mining bioactive secondary metabolites from marine Streptomyces to provide theoretical reference for the development of marine drugs.Methods F2 medium was used for fermentation of Streptomyces to accumulate secondary metabolites.The crude extract was separated by ethyl acetate extraction,reverse phase silica gel chromatography and semi-preparative high performance liquid chromatography(HPLC).The structures of compounds were determined by MS analysis and NMR analysis as well as comparison with published literatures,and all compounds were tested for antibacterial activity.Bioinformatics methods were used to predict and annotate the biosynthetic gene clusters of Streptomyces sp.S063 genome.Results Three alnumycins compounds alnumycin A(1),alnumycin D(2)and alnumycin C(3),one anthraquinone compound K1115A(4)were isolated from marine Streptomyces sp.S063.Compounds 1-3 showed moderate inhibitory activities against Staphylococcus aureus CCARM 3090.The biosynthetic gene cluster of alnumycin(alm)was mapped from Streptomyces sp.S063 by bioinformatics analysis and the biosynthetic pathway of alnumycins in Streptomyces sp.S063 was proposed in combination with the literature.Conclusion A class of compounds with wide range of biological activities were excavated from marine Streptomyces sp.S063,which have potential medicinal value.At the same time,the biological value contained in marine Streptomyces sp.S063 needs to be further explored.
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