In vitro studies on the anti-enterovirus 71 activity of ι-carrageenan
Objective In vitro studies on the anti-enterovirus 71 activity of the compound ι-carrageenan of marine origin.Methods CPE inhibition assay to evaluate the activity of ι-carrageenan against enterovirus 71,CPE assay to verify the mode of action and cytotoxicity of ι-carrageenan,combined with empty spot assay,Western blot assay and real-time fluorescence quantitative PCR assay for corroboration.Co-IP assay was used to verify its target of action.Results ι-carrageenan has a highly efficient and low-toxic anti-enterovirus 71 activity and is most effective in Vero cells with a concentration of inhibitory 50％(IC50)of 6.29 μg/mL and a concentration of cytotoxicity 50％(CC50)greater than 1 mg/mL.Experiments with different modes of action and different duration of action showed that ι-carrageenan acted mainly in the post-adsorption phase of the virus,and 0-2 h administration could significantly inhibit viral VP1 protein expression.In addition,ι-carrageenan could bind to the viral VP1 protein and thus affect virus-infected cells.Conclusion The good in vitro anti-enterovirus 71 activity of ι-carrageenan provides insights into the discovery and modification of new glycoconjugates of marine origin and offers new ideas for the search for new anti-enterovirus 71 drugs.
anti-enterovirus 71ι-carrageenan3C proteinVP1 protein
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