ι-卡拉胶体外抗肠道病毒71型活性研究
In vitro studies on the anti-enterovirus 71 activity of ι-carrageenan
贺福杰 1马骁尧 1徐灿 1吴瑾瑾 1王世欣 2王伟1
作者信息
- 1. 中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛 266003
- 2. 中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛 266003;青岛海洋生物医药研究院,山东青岛 266071
- 折叠
摘要
目的 对海洋来源的化合物ι-卡拉胶进行体外抗肠道病毒71型活性研究.方法 细胞病变效应(CPE)抑制实验评价ι-卡拉胶抗肠道病毒71型的活性,CPE实验探究ι-卡拉胶的细胞毒性.通过空斑实验、Western blot实验、实时荧光定量PCR实验探究ι-卡拉胶的抗病毒活性.利用Co-IP实验探究其作用靶点.结果 ι-卡拉胶具有高效低毒的抗肠道病毒71型活性,在Vero细胞中效果最好,半抑制浓度(IC50)为6.29 μg/mL,半数毒性浓度(CC50)大于1 mg/mL,不同作用方式和不同作用时间实验表明,ι-卡拉胶主要起作用于病毒吸附后阶段,且0～2 h给药可以显著抑制病毒VP1蛋白表达.此外,ι-卡拉胶可以与病毒VP1蛋白结合,从而影响病毒感染细胞.结论 ι-卡拉胶具有良好的体外抗肠道病毒71型活性,为发现和改造新的海洋来源的糖类化合物提供了借鉴,为寻找新的抗肠道病毒71型药物提供了新思路.
Abstract
Objective In vitro studies on the anti-enterovirus 71 activity of the compound ι-carrageenan of marine origin.Methods CPE inhibition assay to evaluate the activity of ι-carrageenan against enterovirus 71,CPE assay to verify the mode of action and cytotoxicity of ι-carrageenan,combined with empty spot assay,Western blot assay and real-time fluorescence quantitative PCR assay for corroboration.Co-IP assay was used to verify its target of action.Results ι-carrageenan has a highly efficient and low-toxic anti-enterovirus 71 activity and is most effective in Vero cells with a concentration of inhibitory 50％(IC50)of 6.29 μg/mL and a concentration of cytotoxicity 50％(CC50)greater than 1 mg/mL.Experiments with different modes of action and different duration of action showed that ι-carrageenan acted mainly in the post-adsorption phase of the virus,and 0-2 h administration could significantly inhibit viral VP1 protein expression.In addition,ι-carrageenan could bind to the viral VP1 protein and thus affect virus-infected cells.Conclusion The good in vitro anti-enterovirus 71 activity of ι-carrageenan provides insights into the discovery and modification of new glycoconjugates of marine origin and offers new ideas for the search for new anti-enterovirus 71 drugs.
关键词
抗肠道病毒71型/ι-卡拉胶/3C蛋白/VP1蛋白Key words
anti-enterovirus 71/ι-carrageenan/3C protein/VP1 protein引用本文复制引用
出版年
2024
NETL
NSTL
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