Study on the anti-Herpes simplex virus type-2 in vitro of polysaccharide sulfate from marine sourse
Objective To study the anti-Herpes simplex virus type-2 in vitro of polysaccharide sulfate from marine sourse.Methods CPE inhibition test,Western blot test and MTT assay were used to evaluate the anti-HSV-2 activity and cytotoxicity of SPM in vitro,respectively.In addition,the specific action mode and specific action time after adsorption were explored by Western blot test.Finally,plaque reduction,membrane fusion inhibition and RT-PCR experiments were used to explore its preliminary target.Results SPM has good anti-HSV-2 activity in vitro,the IC50 in Vero cells was(16.1±1.8)μg/mL and the SI value was greater than 62.1.In addition,the specific mode of action and the specific stage of action after adsorption showed that SPM could directly interact with the virus to inactivate the virus,and could also affect the adsorption,endocytosis and proliferation of the virus to play an anti-HSV-2 role.Conclusion SPM has good anti-HSV-2 activity in vitro,with the characteristics of high efficiency and low toxicity,which is different from the existing anti-Herpes simplex virus drugs,and provides new research ideas and drug research and development direction for the prevention and treatment of herpes virus infection.
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