Objective To investigate the effect of Naringin-induced autophagy on the expression of inflammatory factors in serum and chondrocytes in a rat model of osteoarthritis.Methods From November 2022 to December 2022,30 healthy Sprague-Dawley rats were randomly divided into a control group,a model group,and low-dose,medium-dose,and high-dose experimental groups,with six rats in each group.The model group and the low-dose,medium-dose,and high-dose experimental groups underwent resection of the medial meniscus and anterior cruciate ligament of the right knee to establish a rat model of osteoarthritis.The control group underwent a sham operation.During the modeling period,the low-dose,medium-dose,and high-dose experimental groups were administered Naringin at 25,50,and 100 mg/kg per day,respectively,while the control group and model group received an equal volume of 0.9％sodium chloride injection.After successful modeling,the Osteoarthritis Research Society International(OARSI)score and synovial score were used to assess the severity of osteoarthritis.The levels of interleukin(IL)-1β,IL-6,and tumor necrosis factor(TNF)-α in serum and chondrocytes were determined using an enzyme-linked immunosorbent assay.The mRNA expressions of IL-1β,IL-6,and TNF-α in chondrocytes were detected by reverse transcription-polymerase chain reaction.The changes in autophagy activity and the AKT/mTOR signaling pathway in chondrocytes were detected by western blotting.Results The OARSI scores for the control,model,and low-dose,medium-dose,and high-dose experimental groups were(0.80±0.75)points,(9.40±1.02)points,(8.20±1.33)points,(6.80±1.17)points,and(4.60±1.50)points,respectively.The synovial scores for these groups were(0.40±0.49)points,(3.80±0.75)points,(3.40±0.49)points,(2.20±0.98)points,and(1.60±0.80)points,respectively.The serum levels of IL-1β in these groups were(186.48±50.31)ng/L,(817.43±66.99)ng/L,(533.42±45.67)ng/L,(462.90±21.43)ng/L,and(396.64±24.66)ng/L,respectively.IL-6 levels in these groups were(448.25±89.42)ng/L,(1 762.49±171.95)ng/L,(1 517.08±83.87)ng/L,(1 019.78±103.32)ng/L,and(819.42±169.37)ng/L,respectively.The TNF-α levels in these groups were(419.67±60.99)ng/L,(1 287.40±184.68)ng/L,(948.73±87.82)ng/L,(860.55±102.21)ng/L,and(726.95±15.65)ng/L,respectively.The OARSI scores,synovial scores,serum levels of IL-1β,IL-6,and TNF-α,as well as the protein expressions of phosphorylated AKT(p-AKT)and phosphorylated mammalian target of rapamycin(p-mTOR)in chondrocytes,were significantly higher in the model group compared with the control group(all P＜0.05).In contrast,the OARSI scores,synovial scores,serum levels of IL-1β,IL-6,and TNF-α,as well as the protein expressions of p62,p-Akt,and p-mTOR in chondrocytes,were significantly lower in the high-dose experimental group compared with the model group(all P＜0.05).However,the protein expression of LC3-Ⅱ in chondrocytes was significantly higher in the high-dose experimental group compared with the model group(P＜0.05).Conclusion Naringin can inhibit osteoarthritis in a rat model of osteoarthritis by inhibiting the Akt/mTOR signaling pathway,activating autophagy,and reducing the secretion of inflammatory factors.
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