Mechanism of lncRNA HAGLR promoting ovarian cancer cell growth and epithelial-mesenchymal transition
Objective To investigate the effect of long noncoding RNA Homeobox D gene cluster antisense growth-associated long noncoding RNA(lncRNA HAGLR)on ovarian cancer cell growth and epithelial-mesenchymal transition(EMT)by regulating nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing protein 3(NLRP3)inflammasome.Methods The normal ovarian cell IOSE-80(IOSE-80 group)and the ovarian cancer cell A2780(A2780 group)were cultured.Then A2780 cells were randomly divided into the lncRNA HAGLR silencing group(siHAGLR group),silencing negative control group(siNC group),and siHAGLR combined with NLRP3 inhibitor MCC950 treatment group(siHAGLR+MCC950 group).The expression of lncRNA HAGLR was detected by qRT-PCR.The expressions of NLRP3 inflammasome-related proteins NLRP3,caspase-1,ASC and EMT-related proteins Vimentin,Snail1,α-SMA,Twist1 were detected by Western blot.CCK-8 assay was used to detect the proliferation activity of A2780 cells;Transwell assay was used to detect cell migration and invasion ability of A2780 cells.Cell clone formation assay was used to detect the growth ability of A2780 cells.TUNEL staining was used to detect the apoptosis of A2780 cells.Results Compared with the IOSE-80 group,the expressions of lncRNA HAGLR,Vimentin,Snail1,α-SMA,Twist1 in the A2780 group were all up-regulated(P<0.05),while the expressions of NLRP3,caspase-1,and ASC were all down-regulated(P<0.05).Compared with the siNC group,the expressions of lncRNA HAGLR,Vimentin,Snail1,α-SMA,Twist1 in the siHAGLR group were all down-regulated(P<0.05),while the expressions of NLRP3,caspase-1,and ASC were all up-regulated(P<0.05),the cell proliferation rate,number of cell clones,migration and invasion were significantly reduced(P<0.05),while the cell apoptosis number was increased(P<0.05).Compared with the siHAGLR group,there was no significant change in the expression of lncRNA HAGLR in the siHAGLR+MCC950 group(P>0.05),while the expressions of Vimentin,Snail1,α-SMA,Twist1 were all up-regulated(P<0.05),while the expressions of NLRP3,caspase-1,and ASC were all down-regulated(P<0.05),the the cell proliferation rate,number of cell clones,migration and invasion were all significantly increased(P<0.05),while the cell apoptosis number was decreased(P<0.05).Conclusion lncRNA HAGLR promotes the growth and EMT of ovarian cancer cells by inhibiting NLRP3 inflammasome.
long noncoding RNA Homeobox D gene cluster antisense growth-associated long noncoding RNAnucleotide-binding oligomerization domain-like receptor family pyrin domain-containing protein 3inflammasomeovarian cancer cellsepithelial-mesenchymal transition
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