首页|lncRNA FTX通过靶向miR-590调控膀胱癌细胞的增殖、迁移和侵袭

lncRNA FTX通过靶向miR-590调控膀胱癌细胞的增殖、迁移和侵袭

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目的 探究lncRNA FTX在膀胱癌中的表达及其对肿瘤细胞增殖、迁移、侵袭的影响和相关机制.方法 RT-qPCR检测34例膀胱癌患者癌组织和癌旁组织、人膀胱癌细胞系(5637、RT4、T24和UMUC3)及人输尿管上皮细胞系(SV-HUC-1)中lncRNA FTX和miR-590表达.将沉默lncRNA FTX表达的慢病毒载体(sh-FTX)和阴性对照(sh-NC)分别转染RT4和T24细胞,并记为sh-FTX组和sh-NC组,共转染sh-NC与miR-NC的细胞为sh-NC+miR-NC组,共转染sh-NC与miR-590 inhibitor的细胞为sh-NC+miR-590 inhibitor组,共转染sh-FTX与miR-NC的细胞为sh-FTX+miR-NC组,共转染sh-FTX与miR-590 inhibitor的细胞为sh-FTX+miR-590 inhibitor组.CCK-8检测RT4和T24细胞的增殖率,细胞划痕实验检测RT4和T24细胞的迁移能力,Transwell检测细胞的侵袭能力.Pearson相关系数分析膀胱癌组织中lncRNA FTX表达与miR-590表达的相关性.生物信息学技术和双荧光素酶基因报告实验验证lncRNA FTX和miR-590的调控关系.结果 与癌旁组织相比,癌组织中lncRNA FTX的表达明显升高(P<0.01),而miR-590的表达水平显著降低(P<0.01);在膀胱癌组织中miR-590表达与lncRNA FTX表达呈负相关.与SV-HUC-1细胞相比,5637、RT4、T24及UMUC3细胞中lncRNA FTX的表达均明显升高(P<0.05).与sh-NC组相比,sh-FTX组RT4和T24细胞的增殖率、迁移和侵袭能力均明显降低(P<0.05).lncRNA FTX可靶向抑制miR-590表达.与sh-NC+miR-NC组相比,sh-NC+miR-590 inhibitor组RT4和T24细胞增殖率、迁移和侵袭能力均显著升高(P<0.01),sh-FTX+miR-NC组RT4和T24细胞增殖率、迁移和侵袭能力明显降低(P<0.01),sh-FTX+miR-590 inhibitor组RT4和T24细胞增殖率、迁移和侵袭能力无明显变化(P>0.05);与sh-FTX+miR-NC组相比,sh-FTX+miR-590 inhibitor组RT4和T24细胞的增殖率、迁移和侵袭能力均明显升高(P<0.05).结论 lncRNA FTX在膀胱癌组织及细胞中表达升高,下调其表达可能通过调控miR-590表达抑制膀胱癌的进展.
lncRNA FTX regulating proliferation,migration and invasion of bladder cancer cells by targeting miR-590
Objective To investigate the expression of lncRNA FTX in bladder cancer and its effects on tumor cell proliferation,migration and invasion and related mechanisms.Methods RT-qPCR was used to detect the expression level of lncRNA FTX and miR-590 in bladder cancer tissues and para-cancer tissues of 34 cases,human bladder cancer cell lines(5637,RT4,T24 and UMUC3)and normal ureteral epithelial cell lines(SV-HUC-1).RT4 and T24 cells were respectively transfected with lentiviral vector for silencing lncRNA FTX expression(sh-FTX)and its negative control(sh-NC),and recorded as the sh-FTX group and the sh-NC group,the cells co-transfected with sh-NC and miR-NC were as the sh-NC+miR-NC group,the cells co-transfected with sh-NC and miR-590 inhibitor were as the sh-NC+miR-590 inhibitor group,the cells co-transfected with sh-FTX and miR-NC were as the sh-FTX+miR-NC group,and the cells co-transfected with sh-FTX and miR-590 inhibitor were as the sh-FTX+miR-590 inhibitor group.The proliferation rate of RT4 and T24 cells was detected by CCK-8 method,the migration ability of RT4 and T24 cells was detected by cell scratch wound healing assay,and the invasion ability of cells was detected by Transwell.Pearson correlation coefficient analysis was used to analyze the correlation between lncRNA FTX expression and miR-590 expression in bladder cancer tissues.Bioinformatics technique and dual luciferase gene reporter assay were used to verify the regulatory relationship between lncRNA FTX and miR-590.Results Compared with the para-cancer tissues,the expression of lncRNA FTX in cancer tissues was significantly increased(P<0.01),while the expression level of miR-590 was significantly decreased(P<0.01);miR-590 expression was negatively correlated with lncRNA FTX expression in bladder cancer tissues.Compared with SV-HUC-1 cell,the expression of lncRNA FTX in the 5637,RT4,T24 and UMUC3 cells was significantly increased(P<0.05).Compared with the sh-NC group,the proliferation rate and migration and invasion abilities of RT4 and T24 cells in the sh-FTX group were significantly reduced(P<0.05).lncRNA FTX had targeted inhibition effect on miR-590 expression.Compared with the sh-NC+miR-NC group,the proliferation rate,and migration and invasion abilities of RT4 and T24 cells in the sh-NC+miR-590 inhibitor group were significantly increased(P<0.01),the proliferation rate,and migration and invasion abilities of RT4 and T24 cells in the sh-FTX+miR-NC group were significantly decreased(P<0.01),and the proliferation rate,and migration and invasion abilities of RT4 and T24 cells in the sh-FTX+miR-590 inhibitor group were not significantly changed(P>0.05).Compared with the sh-FTX+miR-NC group,the proliferation rate,and migration and invasion abilities of RT4 and T24 cells in the sh-FTX+miR-590 inhibitor group were significantly increased(P<0.05).Conclusion The expression of lncRNA FTX is increased in bladder cancer tissues and cells,and down-regulation of its expression may inhibit the progression of bladder cancer by regulating the expression of miR-590.

bladder cancerlncRNA FTXmiR-590migrationinvasionmiR-590

刘军、杜恒、马彬

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新疆医科大学第二附属医院泌尿外科,新疆 乌鲁木齐 830063

膀胱癌 lncRNA FTX miR-590 迁移 侵袭 miR-590

新疆维吾尔自治区自然科学基金

2021D01C364

2024

局解手术学杂志
重庆市解剖学会,第三军医大学

局解手术学杂志

CSTPCD
影响因子:1.063
ISSN:1672-5042
年,卷(期):2024.33(7)
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