Expression of long non-coding RNA PVT1 and bioinformatics prediction of its target gene in ovarian cancer
Objective To detect the expression of long non-coding RNA plasma-cytoma variant translocation gene 1(lncRNA PVT1)in ovarian cancer tissues and predict its target gene,and then conduct bioinformatics analysis of the target gene.Methods The cancer tissues and incisal margin normal tissues of 125 patients with ovarian cancer who underwent surgical treatment in our hospital from June 2019 to December 2019 were collected.The expression of lncRNA PVT1 in cancer tissues and incisal margin normal tissues was detected by RT-PCR.The expression of lncRNA PVT1 in ovarian cancer tissues of patients with different clinicopathological characteristics was compared.The lncRNA PVT1 target genes were screened out by the online tools Anolnc2 database,GEPIA2 database,and UALCAN database to obtain the intersection.DAVID database was used to analyze the GO function and KEGG pathway enrichment of the screened lncRNA PVT1 target gene.Results Compared with incisal margin normal tissues,the relative expression of lncRNA PVT1 in cancer tissues significantly increased(P<0.05).The relative expression of lncRNA PVT1 in cancer tissues of ovarian patients with TNM stage Ⅲ to Ⅳ,poorly differentiated or undifferentiation were higher than those of ovarian patients with TNM stage Ⅰ to Ⅱ,moderately or well differentiated,respectively(P<0.05).There were 44 target genes of lncRNA PVT1.GO function analysis found that the target gene functions were mainly enriched in cell cycle regulation,transcription regulation of RNA polymerase Ⅱ promoter,transcription factor activity,lysine oxidase activity,extracellular matrix,cytoplasm,etc.KEGG pathway analysis showed that the pathway it participated in were mainly enriched in cancer-related signal pathway,transforming growth factor-β(TGF-β)signal pathway,extracellular matrix receptor interaction,cyclic guanosine phosphate(cGMP)-protein kinase G(PKG)signal pathway,phosphatidylinositol 3-kinases(PI3K)-RAC-alpha serine/threonine-protein kinase(AKT)signal pathway,etc.Conclusion The expression of lncRNA PVT1 is up-regulated in ovarian cancer tissues.Its target genes are enriched in multiple biological processes such as cell components,molecular functions,biological regulation and tumor-related signaling pathways such as TGF-β,CGMP-PKG.lncRNA PVT1 plays a role in ovarian cancer by regulating the expression of target genes.
万方数据
维普
